Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies.
Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies.
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DOI：
10.1002/jev2.12397
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发表时间：
2024-01
影响因子：
16
通讯作者：
中科院分区：
文献类型：
--
作者：
脑脊液（CSF）是一种来源于血浆的清澈透明的液体，可保护大脑和脊髓免受机械冲击，提供浮力，清除代谢废物并将细胞外成分运送到大脑中的偏远部位。由于与大脑和脊髓接触，CSF是中枢神经系统（CNS）研究中信息量最大的生物流体。除了其他组分之外，CSF还含有细胞外囊泡（EV），其携带生物活性货物（例如，脂质、核酸、蛋白质），并且可以在CNS内和CNS外具有生物学功能。因此，CSF EV可能充当CNS中通信的介导者和贡献者。因此，它们作为CNS疾病的生物标志物的潜力激发了对CSF EV研究的极大兴趣和关注。然而，相对于其他生物液体中的EV研究，CSF EV的研究存在独特的挑战，包括CSF采集的侵入性、有限的CSF体积以及与血浆相比CSF中EV的数量较低。在此，国际细胞外囊泡学会CSF工作组的目标是通过提供CSF EV研究的当前报告和最佳实践以及建议和报告指南，促进CSF EV研究的重现性。为了实现这一目标，我们创建并向ISEV成员分发了一项全球调查，以评估CSF EV的“最佳实践”方法，然后对CSF EV出版物进行了详细的文献综述，用于策划方法和资源。基于对调查的回复和来自出版物的策划信息，CSF工作组在此提供了建议和报告指南，以促进CSF EV研究在七个领域的再现性：（i）CSF收集、处理和储存;（ii）CSF EV分离/浓缩;（iii）CSF EV大小和数量测量;（iv）CSF EV蛋白研究;（v）CSF EV RNA研究（vi）CSF EV组学研究和（vii）CSF EV功能研究。
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS. Thus, CSF EVs likely serve as both mediators of and contributors to communication in the CNS. Accordingly, their potential as biomarkers for CNS diseases has stimulated much excitement for and attention to CSF EV research. However, studies on CSF EVs present unique challenges relative to EV studies in other biofluids, including the invasive nature of CSF collection, limited CSF volumes and the low numbers of EVs in CSF as compared to plasma. Here, the objectives of the International Society for Extracellular Vesicles CSF Task Force are to promote the reproducibility of CSF EV studies by providing current reporting and best practices, and recommendations and reporting guidelines, for CSF EV studies. To accomplish this, we created and distributed a world‐wide survey to ISEV members to assess methods considered ‘best practices’ for CSF EVs, then performed a detailed literature review for CSF EV publications that was used to curate methods and resources. Based on responses to the survey and curated information from publications, the CSF Task Force herein provides recommendations and reporting guidelines to promote the reproducibility of CSF EV studies in seven domains: (i) CSF Collection, Processing, and Storage; (ii) CSF EV Separation/Concentration; (iii) CSF EV Size and Number Measurements; (iv) CSF EV Protein Studies; (v) CSF EV RNA Studies; (vi) CSF EV Omics Studies and (vii) CSF EV Functional Studies.
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影响因子：
3.9
作者：
Akers, Johnny C.;Ramakrishnan, Valya;Kim, Ryan;Phillips, Shirley;Kaimal, Vivek;Mao, Ying;Hua, Wei;Yang, Isaac;Fu, Chia-Chun;Nolan, John;Nakano, Ichiro;Yang, Yuanfan;Beaulieu, Martin;Carter, Bob S.;Chen, Clark C.
通讯作者：
Chen, Clark C.
DOI：
10.1016/j.jalz.2014.02.004
发表时间：
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期刊：
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影响因子：
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