miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients.
miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients.
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DOI：
10.1007/s11060-015-1784-3
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发表时间：
2015-06
影响因子：
3.9
通讯作者：
Chen, Clark C.
中科院分区：
文献类型：
--
作者：
Akers, Johnny C.;Ramakrishnan, Valya;Kim, Ryan;Phillips, Shirley;Kaimal, Vivek;Mao, Ying;Hua, Wei;Yang, Isaac;Fu, Chia-Chun;Nolan, John;Nakano, Ichiro;Yang, Yuanfan;Beaulieu, Martin;Carter, Bob S.;Chen, Clark C.
来自血浆或脑脊液（CSF）的细胞外囊泡（EV）的分析已成为胶质母细胞瘤患者治疗监测的一个有前途的生物标志物平台。然而，这些临床标本中EV的各种亚群的含量仍然不清楚。在这里，我们表征了来自胶质母细胞瘤患者血清和脑脊液的EV中miRNA种类的相对丰度。EV分离自成胶质细胞瘤细胞系以及成胶质细胞瘤患者的血浆和CSF。通过2，000 ×g（20 min）离心清除细胞碎片后，以10，000 ×g沉淀30 min，分离微泡亚群。通过以120，000 ×g（120 min）沉淀微泡上清液来分离外泌体亚群。进行qRT-PCR以检查miR-21、miR-103、miR-24和miR-125的分布。在选择的胶质母细胞瘤CSF样品中进行总体miRNA谱分析。在血浆和细胞系来源的EV中，外泌体和微泡中miRNA的相对丰度是高度可变的。在一些标本中，大多数miRNA种类被发现在外来体中，而在其他标本中，它们被发现在微泡中。相比之下，CSF外泌体相对于CSF微泡富集miRNA。在CSF中，平均每150- 25，000个EV中有一个miRNA分子。来自临床生物流体的大多数EV缺乏miRNA含量。血浆外泌体或微泡中miRNA种类的相对分布是不可预测的。相反，CSF外泌体是携带miRNA的主要EV隔室。
Analysis of extracellular vesicles (EVs) derived from plasma or cerebrospinal fluid (CSF) has emerged as a promising biomarker platform for therapeutic monitoring in glioblastoma patients. However, the contents of the various subpopulations of EVs in these clinical specimens remain poorly defined. Here we characterize the relative abundance of miRNA species in EVs derived from the serum and cerebrospinal fluid of glioblastoma patients. EVs were isolated from glioblastoma cell lines as well as the plasma and CSF of glioblastoma patients. The microvesicle subpopulation was isolated by pelleting at 10,000×g for 30 min after cellular debris was cleared by a 2,000×g (20 min) spin. The exosome subpopulation was isolated by pelleting the microvesicle supernatant at 120,000×g (120 min). qRT-PCR was performed to examine the distribution of miR-21, miR-103, miR-24, and miR-125. Global miRNA profiling was performed in select glioblastoma CSF samples. In plasma and cell line derived EVs, the relative abundance of miRNAs in exosome and microvesicles were highly variable. In some specimens, the majority of the miRNA species were found in exosomes while in other, they were found in microvesicles. In contrast, CSF exosomes were enriched for miRNAs relative to CSF microvesicles. In CSF, there is an average of one molecule of miRNA per 150-25,000 EVs. Most EVs derived from clinical biofluids are devoid of miRNA content. The relative distribution of miRNA species in plasma exosomes or microvesicles is unpredictable. In contrast, CSF exosomes are the major EV compartment that harbor miRNAs.
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影响因子：
16
作者：
Crescitelli R;Lässer C;Szabó TG;Kittel A;Eldh M;Dianzani I;Buzás EI;Lötvall J
通讯作者：
Lötvall J
影响因子：
4
作者：
Salido-Guadarrama I;Romero-Cordoba S;Peralta-Zaragoza O;Hidalgo-Miranda A;Rodríguez-Dorantes M
通讯作者：
Rodríguez-Dorantes M
DOI：
10.1083/jcb.200508014
发表时间：
2006-03-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Booth AM;Fang Y;Fallon JK;Yang JM;Hildreth JE;Gould SJ
通讯作者：
Gould SJ
影响因子：
3.7
作者：
BASS, DM;BAYLOR, M;UPADHYAYULA, U
通讯作者：
UPADHYAYULA, U
影响因子：
5.2
作者：
Joshi, Kaushal;Banasavadi-Siddegowda, Yeshavanth;Mo, Xiaokui;Kim, Sung-Hak;Mao, Ping;Kig, Cenk;Nardini, Diana;Sobol, Robert W.;Chow, Lionel M. L.;Kornblum, Harley I.;Waclaw, Ronald;Beullens, Monique;Nakano, Ichiro
通讯作者：
Nakano, Ichiro