Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling.
Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling.
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DOI：
10.1007/s00438-010-0592-x
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Henry SA
中科院分区：
文献类型：
--
作者：
Villa-García MJ;Choi MS;Hinz FI;Gaspar ML;Jesch SA;Henry SA
芽殖酵母中的肌醇营养缺陷型（Ino−表型）通常与 INO1 和其他参与脂质代谢的基因的失调有关。为了鉴定在缺乏肌醇的情况下生长所必需的所有非必需酵母基因，我们对在一种或多种生长条件下表现出Ino-表型的缺失突变体进行了全基因组表型筛选。我们报告了 419 个基因的鉴定，其中包括 385 个以前未报道过的基因，这些基因在删除后表现出这种表型。已鉴定的基因涉及广泛的细胞过程，但特别富含影响转录、蛋白质修饰、膜运输、多种应激反应和脂质代谢的基因。在参与应激反应的 Ino− 突变体中，许多突变体表现出在高温和/或培养基中存在胆碱时增强的表型。讨论了肌醇在调节脂质代谢和应激反应信号传导中的作用。
Inositol auxotrophy (Ino− phenotype) in budding yeast has classically been associated with misregulation of INO1 and other genes involved in lipid metabolism. To identify all non-essential yeast genes that are necessary for growth in the absence of inositol, we carried out a genome-wide phenotypic screening for deletion mutants exhibiting Ino− phenotypes under one or more growth conditions. We report the identification of 419 genes, including 385 genes not previously reported, which exhibit this phenotype when deleted. The identified genes are involved in a wide range of cellular processes, but are particularly enriched in those affecting transcription, protein modification, membrane trafficking, diverse stress responses, and lipid metabolism. Among the Ino− mutants involved in stress response, many exhibited phenotypes that are strengthened at elevated temperature and/or when choline is present in the medium. The role of inositol in regulation of lipid metabolism and stress response signaling is discussed.
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影响因子：
4.8
作者：
BACHHAWAT, N;OUYANG, QA;HENRY, SA
通讯作者：
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通讯作者：
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