Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers.
Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers.
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DOI：
10.3390/cancers8090085
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发表时间：
2016-09-09
期刊：
影响因子：
5.2
通讯作者：
Stack MS
中科院分区：
文献类型：
--
作者：
Shah SS;Senapati S;Klacsmann F;Miller DL;Johnson JJ;Chang HC;Stack MS
由人乳头瘤病毒（HPV）引起的粘液瘤感染是越来越多的恶性肿瘤的原因，主要以宫颈癌和口咽鳞状细胞癌为代表。由于该病毒的流行、感染的持续性和长潜伏期，需要新的和低成本的方法来进行有效的人群水平的筛查和监测。我们回顾了宫颈癌和口腔癌的筛查方法，以及市售的HPV DNA检测技术。然后，我们描述了正在进行的开发的微流体核酸为基础的生物传感器，以评估循环的主机microRNA的致癌HPV感染的反应。我们的目标是开发一个理想的筛选平台，是低成本，便携式，易于使用，具有适当的信号稳定性，灵敏度和特异性。提供了用于样品裂解、预处理和浓缩以及多重核酸检测的技术的进展。这些设备的持续发展为在资源匮乏的环境中进行癌症筛查、即时诊断和自我筛查以及监测疫苗接种或手术治疗的反应提供了机会。
Mucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-cost methods are needed for effective population level screening and monitoring. We review established methods for screening of cervical and oral cancer as well as commercially-available techniques for detection of HPV DNA. We then describe the ongoing development of microfluidic nucleic acid-based biosensors to evaluate circulating host microRNAs that are produced in response to an oncogenic HPV infection. The goal is to develop an ideal screening platform that is low-cost, portable, and easy to use, with appropriate signal stability, sensitivity and specificity. Advances in technologies for sample lysis, pre-treatment and concentration, and multiplexed nucleic acid detection are provided. Continued development of these devices provides opportunities for cancer screening in low resource settings, for point-of-care diagnostics and self-screening, and for monitoring response to vaccination or surgical treatment.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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