Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip.
Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip.
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DOI：
10.1371/journal.pone.0048329
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Maeda M
中科院分区：
文献类型：
--
作者：
Arata H;Komatsu H;Hosokawa K;Maeda M
microRNA是一种小的非编码单链RNA，其检测是新一代癌症研究的关键课题之一，因为通过microRNA检测可以检测甚至分类人体内的癌症。该报告显示了使用无动力微流体装置进行快速灵敏的microRNA检测，该装置由脱气的聚二甲基硅氧烷驱动，从而无需外部电源。通过夹心杂交检测微小RNA，并通过层流辅助树突状扩增来扩增信号。这种方法使我们能够从0.5 µL样品溶液中检测特定序列的microRNA，检测限为0.5 pM，检测时间为20 min。再加上这种设备的独立性优势，这种方法可能会大大有助于未来的床旁早期癌症诊断。
Detection of microRNAs, small noncoding single-stranded RNAs, is one of the key topics in the new generation of cancer research because cancer in the human body can be detected or even classified by microRNA detection. This report shows rapid and sensitive microRNA detection using a power-free microfluidic device, which is driven by degassed poly(dimethylsiloxane), thus eliminating the need for an external power supply. MicroRNA is detected by sandwich hybridization, and the signal is amplified by laminar flow-assisted dendritic amplification. This method allows us to detect microRNA of specific sequences at a limit of detection of 0.5 pM from a 0.5 µL sample solution with a detection time of 20 min. Together with the advantages of self-reliance of this device, this method might contribute substantially to future point-of-care early-stage cancer diagnosis.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
7.4
作者：
Hosokawa, Kazuo;Omata, Masaki;Maeda, Mizuo
通讯作者：
Maeda, Mizuo
影响因子：
1.6
作者：
Hosokawa, Kazuo;Sato, Takahiro;Maeda, Mizuo
通讯作者：
Maeda, Mizuo
DOI：
10.1073/pnas.0403293101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Liu, CG;Calin, GA;Croce, CM
通讯作者：
Croce, CM
影响因子：
14.9
作者：
Duan D;Zheng KX;Shen Y;Cao R;Jiang L;Lu Z;Yan X;Li J
通讯作者：
Li J