Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis.
Identification of exopolysaccharide-deficient mutants of Mycoplasma pulmonis.
复制标题
肺支原体胞外多糖缺陷突变体的鉴定。
DOI：
10.1111/j.1365-2958.2009.06720.x
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发表时间：
2009-06
影响因子：
3.6
通讯作者：
Dybvig, Kevin
中科院分区：
文献类型：
--
作者：
Daubenspeck, James M.;Bolland, Jeffrey R.;Luo, Wenyi;Simmons, Warren L.;Dybvig, Kevin
荚膜外多糖（EPS）存在于Mollicutes中已有50多年的历史，但没有确凿的数据来支持生物体生产复杂的碳水化合物。肺支原体结合了对末端β连接的半乳糖残基具有特异性的凝集素Griffonia simplicifolia I （GS-I）。从转座子文库中分离出不能产生GS-I结合的EPS的突变体。所有突变体的转座子都位于开放阅读框MYPU_7410或MYPU_7420。预计这些重叠的基因编码一对异二聚体ABC转运蛋白透孔，并可能编码EPS合成新途径的一部分。凝集素亲和层析和气相层析相结合的分析表明，野生型支原体产生由等量的葡萄糖和半乳糖组成的EPS (EPS- 1)，这是突变体所缺乏的。表型分析表明，突变体在玻璃表面形成生物膜的倾向增加，有效定植小鼠肺和气管，但与A549肺细胞系的相关性降低。对这些结果的解释较为混乱的是，缺失EPS- 1的突变体含有n -乙酰氨基葡萄糖的第二EPS （EPS- 2）明显过量生产8倍。
The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over fifty years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal β-linked galactose residues. Mutants that failed to produce the EPS bound by GS-I were isolated from a transposon library. All of the mutants had the transposon located in open reading frame MYPU_7410 or MYPU_7420. These overlapping genes are predicted to code for a heterodimeric pair of ABC transporter permeases and may code for part of a new pathway for synthesis of EPS. Analysis by lectin-affinity chromatography in conjunction with gas chromatography demonstrated that the wild-type mycoplasma produced an EPS (EPS-I) composed of equimolar amounts of glucose and galactose that was lacking in the mutants. Phenotypic analysis revealed that the mutants had an increased propensity to form a biofilm on glass surfaces, colonized mouse lung and trachea efficiently, but had a decreased association with the A549 lung cell line. Confounding the interpretation of these results is the observation that the mutants missing EPS-I had an 8-fold overproduction of an apparent second EPS (EPS-II) containing N-acetylglucosamine.
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DOI：
10.1111/j.1749-6632.1967.tb27658.x
发表时间：
1967-01-01
影响因子：
5.2
作者：
CHU, HP;HORNE, RW
通讯作者：
HORNE, RW
影响因子：
3.1
作者：
DAVIS, JK;THORP, RB;CASSELL, GH
通讯作者：
CASSELL, GH
影响因子：
3.2
作者：
Shen, XJ;Gumulak, J;Dybvig, K
通讯作者：
Dybvig, K
影响因子：
3.1
作者：
Simmons, Warren L.;Dybvig, Kevin
通讯作者：
Dybvig, Kevin
影响因子：
3.1
作者：
Simmons, WL;Dybvig, K
通讯作者：
Dybvig, K