Knockout of the ABCB1 Gene Increases Susceptibility to Emamectin Benzoate, Beta-Cypermethrin and Chlorantraniliprole in Spodoptera frugiperda.
Knockout of the ABCB1 Gene Increases Susceptibility to Emamectin Benzoate, Beta-Cypermethrin and Chlorantraniliprole in Spodoptera frugiperda.
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DOI：
10.3390/insects13020137
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发表时间：
2022-01-27
期刊：
影响因子：
3
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Li Q;Jin M;Yu S;Cheng Y;Shan Y;Wang P;Yuan H;Xiao Y
草地贪夜蛾是一种入侵性鳞翅目害虫，对许多经济作物造成广泛损害。为了控制这种害虫，化学农药的使用变得越来越重要，因为其效率高、成本低且易于机械化操作。然而，尽管农药的有效性已得到证实，但长期使用化学品不仅污染环境，而且会导致田间种群产生抗药性，使害虫防治变得更加困难。在本研究中，我们使用 CRISPR/Cas9 系统生成 ABCB1 敲除菌株，以探索其在确定化学农药或 Bt 毒素敏感性方面的潜在作用。我们比较了用 10 种杀虫剂和 3 种 Bt 蛋白处理的 WT 品系和敲除品系的死亡率，发现在 ABCB1 被敲除后，昆虫对 3 种杀虫剂（甲维盐、高效氯氰菊酯和氯虫苯甲酰胺）显着更敏感。我们的研究结果表明ABCB1与一些农药的解毒代谢密切相关，提示草地贪夜蛾对甲维盐、高效氯氰菊酯和氯虫苯甲酰胺的抗性可能与ABCB1的过度表达有关。这为后续研究草地贪夜蛾的抗性机制奠定了基础。 ATP结合盒转运蛋白B1（ABCB1或P-糖蛋白）已知是哺乳动物多药耐药性的重要参与者，并且也已被证明是几种鳞翅目昆虫中某些杀虫剂的转运蛋白，但该转运蛋白在草地贪夜蛾中的确切功能尚不清楚。在这里，我们使用 CRISPR/Cas9 系统生成了 SfABCB1 敲除草地贪夜蛾菌株，以探索其在确定化学杀虫剂或 Bt 毒素敏感性方面的潜在作用。生测结果显示，与野生型菌株DH19相比，SfABCB1敲除菌株对高效氯氰菊酯、氯虫苯甲酰胺和甲维盐苯甲酸盐的敏感性显着升高，而Cry1Ab、Cry1Fa和Vip3Aa的Bt毒素无变化。我们的结果表明，SfABCB1在草地贪夜蛾对高效氯氰菊酯、氯虫苯甲酰胺和甲维盐苯甲酸盐的敏感性中起重要作用，这表明ABCB1的过表达可能有助于草地贪夜蛾对高效氯氰菊酯、氯虫苯甲酰胺和甲维盐苯甲酸盐的抗性。
Spodoptera frugiperda is an invasive lepidopteran pest that causes extensive damage to many commercial crops. To control this pest, the use of chemical pesticides has become increasingly important because of their high efficiency, low cost and ease of mechanized operations. However, despite the proven effectiveness of pesticides, the long-term use of chemicals not only pollutes the environment, but also leads to resistance in field populations, making pest control more difficult. In this study, we used the CRISPR/Cas9 system to generate an ABCB1 knockout strain to explore its potential role in determining susceptibility to chemical pesticides or Bt toxins. We compared the mortality of WT strains and knockout strains treated with 10 insecticides and three Bt proteins, and found that the insects were significantly more susceptible to the three insecticides (emamectin benzoate, beta-cypermethrin and chlorantraniliprole) after ABCB1 was knocked out. Our results showed that ABCB1 is closely related to the detoxification metabolism of some pesticides, and suggest that the resistance of Spodoptera frugiperda to emamectin benzoate, beta-cypermethrin and chlorantraniliprole may be related to the overexpression of ABCB1. This lays a foundation for a follow-up study on the resistance mechanism of Spodoptera frugiperda. ATP-binding cassette transporter B1 (ABCB1, or P-glycoprotein) is known to be an important participant in multidrug resistance in mammals, and it also has been proved as a transporter for some insecticides in several lepidopteran insects, yet the precise function of this transporter in Spodoptera frugiperda is unknown. Here, we generated a SfABCB1 knockout strain of the S. frugiperda using the CRISPR/Cas9 system to explore its potential roles in determining susceptibility to chemical insecticides or Bt toxins. Bioassay results showed that the susceptibility of SfABCB1 knockout strain to beta-cypermethrin, chlorantraniliprole and emamectin benzoate were significantly increased compared with the wild-type strain DH19, whereas there were no changes to Bt toxins for Cry1Ab, Cry1Fa and Vip3Aa. Our results revealed that SfABCB1 plays important roles in the susceptibility of S. frugiperda to beta-cypermethrin, chlorantraniliprole and emamectin benzoate, and imply that overexpression of ABCB1 may contribute to beta-cypermethrin, chlorantraniliprole and emamectin benzoate resistance in S. frugiperda.
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影响因子：
3.7
作者：
Hawthorne DJ;Dively GP
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1102555108
发表时间：
2011-08-23
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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