HCF-1 promotes cell cycle progression by regulating the expression of CDC42.
HCF-1 promotes cell cycle progression by regulating the expression of CDC42.
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DOI：
10.1038/s41419-020-03094-5
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发表时间：
2020-10-23
影响因子：
9
通讯作者：
Peng X
中科院分区：
文献类型：
--
作者：
Xiang P;Li F;Ma Z;Yue J;Lu C;You Y;Hou L;Yin B;Qiang B;Shu P;Peng X
真核细胞周期涉及一系列高度协调的事件，其中细胞基因组在合成（S）期复制，并且两个所得拷贝中的每一个在有丝分裂（M）期间适当地分离。宿主细胞因子-1（HCF-1）是一种转录辅助调节因子，它对基本的细胞过程（如转录调节和细胞周期进程）至关重要，并与之有关。虽然已经鉴定了一系列HCF-1转录靶点，但很少提供功能线索，特别是染色体分离。我们的研究结果表明，HCF-1通过与CDC 42转录起始位点上游的-881至575区域结合来激活CDC 42的表达，并且HCF-1对CDC 42表达的调节与细胞周期进程相关。自发循环和组成型活性CDC 42突变体（CDC 42 F28 L）的过表达挽救了HCF-1缺失后有丝分裂中的G1期延迟和多核缺陷。因此，这些结果表明，HCF-1通过调节CDC 42的表达来确保适当的细胞周期进程，这表明细胞周期协调的可能机制和典型Rho GTP酶的调节模式。
The eukaryotic cell cycle involves a highly orchestrated series of events in which the cellular genome is replicated during a synthesis (S) phase and each of the two resulting copies are segregated properly during mitosis (M). Host cell factor-1 (HCF-1) is a transcriptional co-regulator that is essential for and has been implicated in basic cellular processes, such as transcriptional regulation and cell cycle progression. Although a series of HCF-1 transcriptional targets have been identified, few functional clues have been provided, especially for chromosome segregation. Our results showed that HCF-1 activated CDC42 expression by binding to the −881 to −575 region upstream of the CDC42 transcription start site, and the regulation of CDC42 expression by HCF-1 was correlated with cell cycle progression. The overexpression of a spontaneously cycling and constitutively active CDC42 mutant (CDC42F28L) rescued G1 phase delay and multinucleate defects in mitosis upon the loss of HCF-1. Therefore, these results establish that HCF-1 ensures proper cell cycle progression by regulating the expression of CDC42, which indicates a possible mechanism of cell cycle coordination and the regulation mode of typical Rho GTPases.
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影响因子：
7
作者：
Michaud J;Praz V;James Faresse N;Jnbaptiste CK;Tyagi S;Schütz F;Herr W
通讯作者：
Herr W
影响因子：
10.5
作者：
Goto, H;Motomura, S;Nishimoto, T
通讯作者：
Nishimoto, T
影响因子：
16.6
作者：
Shilatifard A
通讯作者：
Shilatifard A
DOI：
10.1126/science.1243990
发表时间：
2013-12-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lazarus MB;Jiang J;Kapuria V;Bhuiyan T;Janetzko J;Zandberg WF;Vocadlo DJ;Herr W;Walker S
通讯作者：
Walker S
影响因子：
4.8
作者：
Chou, MM;Masuda-Robens, JM;Gupta, ML
通讯作者：
Gupta, ML