Morphological, genetic and molecular characteristics of barley root hair mutants.
Morphological, genetic and molecular characteristics of barley root hair mutants.
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DOI：
10.1007/s13353-014-0225-x
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发表时间：
2014-11
影响因子：
2.4
通讯作者：
Szarejko, Iwona
中科院分区：
文献类型：
--
作者：
Chmielewska, Beata;Janiak, Agnieszka;Karcz, Jagna;Guzy-Wrobelska, Justyna;Forster, Brian P.;Nawrot, Malgorzata;Rusek, Anna;Smyda, Paulina;Kedziorski, Piotr;Maluszynski, Miroslaw;Szarejko, Iwona
根毛是由特化的表皮细胞（称为毛细胞）管状生长而成。它们影响植物在土壤中的锚定、水和养分的吸收，并且是植物和微生物之间相互作用的场所。对19个代表不同根毛发育阶段的大麦根毛突变体进行了详细的形态和遗传分析。各突变体均为单基因隐性突变。等位性检测表明，9个位点负责收集的突变根毛表型和1-4个突变等位基因的形式被确定在每个位点。建立了根毛形成不同阶段基因间的遗传关系。4个位点的连锁群rhl 1，rhp 1，rhi 1和rhs 1，这已经被定位在染色体7 H，1H，6 H和5 H，分别与新的标记，侧翼的基因在0.16 cM至4.6 cM的距离丰富。三个新基因的染色体位置-两个负责短根毛的发育（rhs 2和rhs 3）和控制不规则根毛模式的基因（rhi 2）-分别定位在染色体6 H，2 H和1H上。一些突变体和它们各自的亲本系之间的agrobotanical参数的比较分析表明，在基因中负责根毛发育的突变对在受控条件下生长的植物的agrobotanical性能没有影响。该突变体库为进一步鉴定控制大麦根毛发育的基因提供了有价值的工具。本文的在线版本（doi：10.1007/s13353-014-0225-x）包含补充材料，可供授权用户使用。
Root hairs are tubular outgrowths of specialized epidermal cells called trichoblasts. They affect anchoring plants in soil, the uptake of water and nutrients and are the sites of the interaction between plants and microorganisms. Nineteen root hair mutants of barley representing different stages of root hair development were subjected to detailed morphological and genetic analyses. Each mutant was monogenic and recessive. An allelism test revealed that nine loci were responsible for the mutated root hair phenotypes in the collection and 1–4 mutated allelic forms were identified at each locus. Genetic relationships between the genes responsible for different stages of root hair formation were established. The linkage groups of four loci rhl1, rhp1, rhi1 and rhs1, which had previously been mapped on chromosomes 7H, 1H, 6H and 5H, respectively, were enriched with new markers that flank the genes at a distance of 0.16 cM to 4.6 cM. The chromosomal position of three new genes – two that are responsible for the development of short root hairs (rhs2 and rhs3) and the gene that controls an irregular root hair pattern (rhi2) – were mapped on chromosomes 6H, 2H and 1H, respectively. A comparative analysis of the agrobotanical parameters between some mutants and their respective parental lines showed that mutations in genes responsible for root hair development had no effect on the agrobotanical performance of plants that were grown under controlled conditions. The presented mutant collection is a valuable tool for further identification of genes controlling root hair development in barley. The online version of this article (doi:10.1007/s13353-014-0225-x) contains supplementary material, which is available to authorized users.
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影响因子：
4.3
作者：
Kwasniewski, Miroslaw;Janiak, Agnieszka;Szarejko, Iwona
通讯作者：
Szarejko, Iwona
影响因子：
7.2
作者：
Caldwell, DG;McCallum, N;Waugh, R
通讯作者：
Waugh, R
影响因子：
5.1
作者：
Huang, Jin;Kim, Chul Min;Han, Chang-Deok
通讯作者：
Han, Chang-Deok
影响因子：
4.9
作者：
Gahoonia, TS;Nielsen, NE
通讯作者：
Nielsen, NE
影响因子：
5.4
作者：
Kikuchi, S;Taketa, S;Kawasaki, S
通讯作者：
Kawasaki, S