Nanoparticle technology: addressing the fundamental roadblocks to protein biomarker discovery.
Nanoparticle technology: addressing the fundamental roadblocks to protein biomarker discovery.
复制标题
DOI：
10.2174/156652410790963268
复制
发表时间：
2010-03
影响因子：
2.5
通讯作者：
Liotta LA
中科院分区：
文献类型：
--
作者：
Luchini A;Fredolini C;Espina BH;Meani F;Reeder A;Rucker S;Petricoin EF 3rd;Liotta LA
临床相关的生物标志物以极低的浓度存在于血液和体液中，被高丰度的高分子量蛋白质掩盖，并且由于内源性和外源性蛋白酶，在收集和运输期间经常发生降解。由内部亲和诱饵功能化的N-异丙基丙烯酰胺水凝胶核壳组成的纳米颗粒是一种新技术，可以解决疾病生物标志物发现和测量的所有这些关键分析挑战。含有诱饵的核-壳纳米颗粒可以在一个步骤中、在溶液中、在复杂的生物流体（例如血液或尿液）中执行四种功能：a）分子大小筛分，B）完全排除高丰度不需要的蛋白质，c）靶分析物亲和螯合，以及d）完全保护捕获的分析物免于降解。被颗粒隔离的蛋白质分析物的目标类别可以被浓缩在小体积中，以有效地放大（取决于起始样品体积，高达100倍或更高）质谱、蛋白质印迹和免疫测定的灵敏度。用于制造颗粒的材料是经济的、长期稳定的，并且在体液中保持完全可溶，以在几分钟内实现几乎100%捕获所有溶液相靶蛋白。
Clinically relevant biomarkers exist in blood and body fluids in extremely low concentrations, are masked by high abundance high molecular weight proteins, and often undergo degradation during collection and transport due to endogenous and exogenous proteinases. Nanoparticles composed of a N-isopropylacrylamide hydrogel core shell functionalized with internal affinity baits are a new technology that can address all of these critical analytical challenges for disease biomarker discovery and measurement. Core-shell, bait containing, nanoparticles can perform four functions in one step, in solution, in complex biologic fluids (e.g. blood or urine): a) molecular size sieving, b) complete exclusion of high abundance unwanted proteins, c) target analyte affinity sequestration, and d) complete protection of captured analytes from degradation. Targeted classes of protein analytes sequestered by the particles can be concentrated in small volumes to effectively amplify (up to 100 fold or greater depending on the starting sample volume) the sensitivity of mass spectrometry, western blotting, and immunoassays. The materials utilized for the manufacture of the particles are economical, stable overtime, and remain fully soluble in body fluids to achieve virtually 100 percent capture of all solution phase target proteins within a few minutes.
登录
查看更多内容
DOI：
10.1016/0378-4347(96)00006-0
发表时间：
1996-04-26
期刊：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS
影响因子：
--
作者：
Koyama, T;Terauchi, K
通讯作者：
Terauchi, K
影响因子：
3.6
作者：
LORAND, JP;EDWARDS, JO
通讯作者：
EDWARDS, JO
影响因子：
3.5
作者：
Ayache, Saleh;Panelli, Monica;Stroncek, David F.
通讯作者：
Stroncek, David F.
影响因子：
3.5
作者：
Baxter, EJ;Hochhaus, A;Cross, NCP
通讯作者：
Cross, NCP
影响因子：
9.3
作者：
Lowenthal, MS;Mehta, AI;Liotta, LA
通讯作者：
Liotta, LA