Simultaneously screening for methicillin-resistant Staphylococcus aureus and its susceptibility to potentiated penicillins.
Simultaneously screening for methicillin-resistant Staphylococcus aureus and its susceptibility to potentiated penicillins.
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同时筛查耐甲氧西林金黄色葡萄球菌及其对强化青霉素的敏感性。
DOI：
10.1099/jmm.0.001562
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发表时间：
2022
影响因子：
3
通讯作者：
Ba X
中科院分区：
文献类型：
--
作者：
Ba X
引言：我们最近发现临床上很大比例的耐甲氧西林金黄色葡萄球菌（MRSA）分离株对青霉素和克拉维酸（强化青霉素）敏感，包括广泛使用的联合用药，如复方阿莫西林。这些分离株在Iso-Sensitest Agar（伊萨）上对苯唑西林的敏感性也有所增加。假设/缺口声明。这些MRSA分离株在伊萨上对苯唑西林的敏感性增加可用于区分它们与耐药菌株。方法：采用10 μg头孢西丁和1 μg苯唑西林纸片在伊萨上进行双纸片扩散，并对120株全基因组测序的MRSA分离株进行检测。与以前发表的基因型和表型方法的方法的灵敏度进行了比较。此外，根据欧洲抗菌药物敏感性试验委员会（EUCAST）protocol.Results.所有分离株（120/120）在Müller-Hinton琼脂（MHA）上进行双纸片扩散，再次确认为表型MRSA，如头孢西丁纸片扩散试验结果所示。所有具有青霉素和克拉维酸（Pen-Clav）耐药基因型的分离株（40/40）均不受苯唑西林抑制，而77/80（96.3%）具有Pen-Clav敏感基因型的分离株在伊萨上受苯唑西林抑制。 结果表明，采用MHA的EUCAST法能准确鉴定所有MRSA菌株，但不能区分Pen-Clav敏感菌株和耐Pen-Clav菌株。结论：采用伊萨的双纸片扩散法可准确筛选临床MRSA菌株，同时测定其对Pen-Clav的敏感性。快速鉴定可能适合用增强青霉素治疗的MRSA感染。
Introduction.We recently revealed that a significant proportion of clinical methicillin-resistantStaphylococcus aureus(MRSA) isolates are susceptible to pencillins and clavulanic acid (potentiated penicillins), including widely available combinations such as co-amoxiclav. These isolates also showed increased susceptibility to oxacillin on Iso-Sensitest Agar (ISA).Hypothesis/Gap Statement.The increased susceptibility to oxacillin displayed on ISA by these MRSA isolates may be used to distinguish them from the resistant ones.Aim.We aimed to develop a method to simultaneously screen aS. aureusclinical isolate for its susceptibility to methicillin and potentiated penicillins.Methodology.A double-disc diffusion method using 10 µg cefoxitin and 1 µg oxacillin discs on ISA was developed and tested against a panel of 120 whole genome-sequenced MRSA isolates. The sensitivity of the method was compared with that of previously published genotypic and phenotypic methods. In addition, double-disc diffusion was performed for all isolates on Müller–Hinton agar (MHA) following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) protocol.Results.All isolates (120/120) were reconfirmed to be phenotypically MRSA, as indicated by the result of cefoxitin disc diffusion testing. All isolates (40/40) that had a pencillins and clavulanic acid (Pen–Clav)-resistant genotype were not inhibited by oxacillin, while 77/80 (96.3 %) isolates that had a Pen–Clav-susceptible genotype were inhibited by oxacillin on ISA. The results also showed that the EUCAST method using MHA correctly identified all isolates as MRSA but failed to distinguish the Pen–Clav-susceptible isolates from the Pen–Clav-resistant isolates.Conclusions.This double-disc diffusion method using ISA could be used to accurately screen for clinical MRSA isolates and determine their susceptibility to Pen–Clav simultaneously, rapidly identifying MRSA infections that might be suitable for treatment with potentiated penicillins.
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DOI：
10.1093/cid/ciz750
发表时间：
2020
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
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作者：
T. Holland;Joshua S. Davis
通讯作者：
Joshua S. Davis
对 Iso-Sensitest 琼脂上的头孢西丁 30 微克圆片用于检测耐甲氧西林金黄色葡萄球菌的评估。
影响因子：
5.2
作者：
R. Skov;R. Smyth;M. Clausen;A. Larsen;N. Frimodt;B. Olsson;G. Kahlmeter
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影响因子：
3.4
作者：
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影响因子：
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作者：
J. Patel;S. Sharp;S. Novak
通讯作者：
S. Novak
影响因子：
5.2
作者：
Fernandes, CJ;Fernandes, LA;Collignon, P
通讯作者：
Collignon, P