Affinity monolith chromatography: a review of principles and recent analytical applications.
Affinity monolith chromatography: a review of principles and recent analytical applications.
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DOI：
10.1007/s00216-012-6568-4
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发表时间：
2013-03
影响因子：
4.3
通讯作者：
Hage, David S.
中科院分区：
文献类型：
--
作者：
Pfaunmiller, Erika L.;Paulemond, Marie Laura;Dupper, Courtney M.;Hage, David S.
关键词：
亲和整体层析(AMC)是一种使用整体载体和与生物相关的结合剂作为固定相的液相色谱学。AMC是一种对样品中特定目标化合物进行选择性分离、分析或研究的有效方法。本综述讨论了AMC的基本原理以及该方法的最新发展和应用，并着重介绍了过去五年中出现的工作。研究了用于制备AMC柱的各种材料，包括有机整体柱、硅胶整体柱、琼脂糖柱和冷冻柱。这些支撑物已在AMC中用于从传统柱到磁盘、微柱和毛细管的各种格式。许多结合剂也被用于AMC，如抗体、酶、蛋白质、凝集素、固定化金属离子和染料。已报道的这些结合剂在AMC中的应用包括生物亲和层析、免疫亲和层析或免疫提取、固定化金属-离子亲和层析、染料-配体亲和层析、手性分离和生物相互作用研究。例子来自分析化学、药物分析、临床测试和生物技术等领域。文中还讨论了AMC的发展趋势和未来可能的发展方向。
Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically-related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis or studies of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments or applications of this method, with particular emphasis being given to work that has appeared in the last five years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths and cryogels. These supports have been used in AMC for formats that have ranged from traditional columns to disks, microcolumns and capillaries. Many binding agents have also been employed in AMC, such as antibodies, enzymes, proteins, lectins, immobilized metal-ions and dyes. Some applications that have been reported with these binding agents in AMC are bioaffinity chromatography, immunoaffinity chromatography or immunoextraction, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, chiral separations and biointeraction studies. Examples are presented from fields that include analytical chemistry, pharmaceutical analysis, clinical testing and biotechnology. Current trends and possible future directions in AMC are also discussed.
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影响因子：
4.1
作者：
Gustavsson, PE;Larsson, PO
通讯作者：
Larsson, PO
影响因子：
4.1
作者：
Arvidsson, P;Plieva, FM;Mattiasson, B
通讯作者：
Mattiasson, B
DOI：
10.1016/j.jpba.2007.01.030
发表时间：
2007-06-28
影响因子：
3.4
作者：
Calleri, E.;Marrubini, G.;Caccialanza, G.
通讯作者：
Caccialanza, G.
影响因子：
5.8
作者：
Cimen, Duygu;Denizli, Adil
通讯作者：
Denizli, Adil
影响因子：
3.4
作者：
Feng, Shun;Pan, Chensong;Zou, Hanfa
通讯作者：
Zou, Hanfa