Optimization of Lipid Extraction and Determination of Fatty Acid Compositions in Edible Meats of Freshwater and Marine Shrimps

Optimization of Lipid Extraction and Determination of Fatty Acid Compositions in Edible Meats of Freshwater and Marine Shrimps
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淡水和海虾食用肉中脂质提取的优化及脂肪酸组成的测定

DOI:
10.1080/10498850.2017.1323066
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发表时间:
2017-08
影响因子:
1.6
通讯作者:
Zeng XX
Zeng XX
中科院分区:
农林科学4区
文献类型:
--
作者:
Zhou Li;Li Peixuan;Zhao Yuling;Hou Shuang;Cong Baolei;Huang Jun;Ding Yu;Zeng Xiaoxiong;Zeng XX

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摘要应用响应曲面法确定了从虾中提取脂肪的最佳工艺条件。采用Box-Behnken设计,包括提取时间、提取温度、混合溶剂比例等自变量。脂肪的最佳提取条件为石油醚:乙酸乙酯(2.3:1,v/v),提取时间为62min,提取温度为52℃。在此条件下,龙须菜、南美白对虾、日本大管藻和罗氏沼虾的总脂得率分别为4.39%、2.80%、2.98%和3.30%。气相色谱分析表明,罗氏沼虾的多不饱和脂肪酸(PUFA)含量最高(48.28%)。此外,20:5n3(EPA)+22:6n3(DHA)值(26.43%)仅次于E.Superba(31.27%)。这一发现表明,罗氏微囊藻是一种潜在的多不饱和脂肪酸食品补充剂来源。
ABSTRACT Response surface methodology was applied to determine optimal conditions for lipid extraction from shrimp. Box-Behnken Design including independent variables such as extraction time, extraction temperature, and ratio of mixed solvents was used. The optimal conditions for lipids extraction were the use of petroleum ether:ethyl acetate (2.3:1, v/v) with 62 min of extraction at 52ºC. Under the optimal conditions, the extraction yields of lipids from Euphausia superba, Penaeuschinesis, Macrobranchium nipponense, and M. rosenbergii were 4.39, 2.80, 2.98, and 3.30%, respectively. Gas chromatographic analysis showed that M. rosenbergii presented the highest level of polyunsaturated fatty acid (PUFA) (48.28%) among the shrimps studied. In addition, the 20:5n3 (EPA) + 22:6n3 (DHA) value (26.43%) was second only to E. superba (31.27%). The finding suggests that M. rosenbergii is a potential PUFA source for food supplementation.
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