Complete chloroplast genome of a valuable medicinal plant, Huperzia serrata (Lycopodiaceae), and comparison with its congener.
Complete chloroplast genome of a valuable medicinal plant, Huperzia serrata (Lycopodiaceae), and comparison with its congener.
复制标题
DOI：
10.3732/apps.1600071
复制
发表时间：
2016-11
影响因子：
3.6
通讯作者：
Zhang XC
中科院分区：
文献类型：
--
作者：
Guo ZY;Zhang HR;Shrestha N;Zhang XC
在这里，我们报道了重要的药用植物锯缘石杉的叶绿体基因组，并将其与同属植物石杉的叶绿体基因组进行了比较。利用Illumina平台对锯缘海棠叶绿体全基因组进行了测序，并与Geneious版本R9.0.5进行了组装。锯缘海鞘基因组大小为154,176个碱基，GC含量为36.3%。完整的叶绿体基因组包含120个独特基因，包括86个编码基因、4个rRNA基因和30个tRNA基因。与光肩星天牛的叶绿体基因组比较发现，这两个物种的3个高变区(rps16-chlB、ycf12-trnR和ycf1)和252个突变事件包括27个插入/缺失多态和225个单核苷酸多态(SNPs)。在基因编码区共检测到92个SNPs。此外，还发现了18个微卫星位点，可用于系统地理学研究。本文报道了锯缘石杉的完整叶绿体基因组，为进一步研究石杉属药用植物的系统发育、进化和医学研究提供了有价值的基因组资源。
Here we report the complete chloroplast genome of the important medicinal species Huperzia serrata (Lycopodiaceae) and compare it to the chloroplast genome of the congeneric species H. lucidula. The whole chloroplast genome of H. serrata was sequenced using an Illumina platform and assembled with Geneious version R9.0.5. The genome size of H. serrata was 154,176 bp, with 36.3% GC content. The complete chloroplast genome contained 120 unique genes, including 86 coding genes, four rRNA genes, and 30 tRNA genes. Comparison with the chloroplast genome of H. lucidula revealed three highly variable regions (rps16-chlB, ycf12-trnR, and ycf1) between these two species and 252 mutation events including 27 insertion/deletion polymorphisms and 225 single-nucleotide polymorphisms (SNPs). Ninety-two SNPs were identified in the gene-coding regions. In addition, 18 microsatellite sites were found, which can potentially be used in phylogeographic studies. The complete chloroplast genome of H. serrata is reported here, and will be a valuable genome resource for further phylogenetic, evolutionary, and medical studies of medicinal plants in the genus Huperzia.
登录
查看更多内容
影响因子：
2.5
作者：
Lohse, Marc;Drechsel, Oliver;Bock, Ralph
通讯作者：
Bock, Ralph
影响因子：
5.8
作者：
Wyman, SK;Jansen, RK;Boore, JL
通讯作者：
Boore, JL
影响因子：
3.5
作者：
Wolf, PG;Karol, KG;Boore, JL
通讯作者：
Boore, JL
影响因子：
4.1
作者：
Kuo, Li-Yaung;Li, Fay-Wei;Wang, Chun-Neng
通讯作者：
Wang, Chun-Neng
影响因子：
5.6
作者：
Song Y;Dong W;Liu B;Xu C;Yao X;Gao J;Corlett RT
通讯作者：
Corlett RT