Genetically engineered Pseudomonas putida X3 strain and its potential ability to bioremediate soil microcosms contaminated with methyl parathion and cadmium
Genetically engineered Pseudomonas putida X3 strain and its potential ability to bioremediate soil microcosms contaminated with methyl parathion and cadmium
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基因工程恶臭假单胞菌 X3 菌株及其生物修复受甲基对硫磷和镉污染的土壤微生物的潜在能力
DOI：
10.1007/s00253-015-7099-7
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发表时间：
2016-02
影响因子：
5
通讯作者：
Qiaoyun Huang
中科院分区：
文献类型：
--
作者：
Rong Zhang;Xingjian Xu;Wenli Chen;Qiaoyun Huang
通过在恶臭假单胞菌X4（CCTCC：209319）中引入甲基异硫氰酸根（MP）降解基因和增强型绿色荧光蛋白（EGFP）基因，成功地改造了多功能恶臭假单胞菌X3菌株。在液体培养中，工程X3菌株利用MP作为生长的唯一碳源，并在24小时内降解100 mg L（-1）的MP;然而，该菌株没有进一步代谢对硝基苯酚（PNP），MP的中间代谢物。X3基因工程菌对镉、铜、锌、钴的最低抑菌浓度（MIC）与宿主菌无差异。接种X3菌株后，在100 mg MP kg（-1）干土和/或5 mg Cd kg（-1）干土的不同污染土壤微宇宙中，MP降解均得到加速，40 h内MP被完全降解。然而，镉的存在下，在修复的早期阶段略有延迟MP降解。X3菌株在Cd污染土壤中的应用强烈影响Cd形态和固定化Cd的分布，通过降低生物有效性Cd浓度，降低可溶性/交换性Cd和有机结合态Cd。接种的X3菌株还在各种污染的微宇宙中定殖和增殖。研究结果表明，X3工程菌是一种潜在的生物修复剂，在复杂污染环境中显示出竞争优势。
A multifunctional Pseudomonas putida X3 strain was successfully engineered by introducing methyl parathion (MP)-degrading gene and enhanced green fluorescent protein (EGFP) gene in P. putida X4 (CCTCC: 209319). In liquid cultures, the engineered X3 strain utilized MP as sole carbon source for growth and degraded 100 mg L(-1) of MP within 24 h; however, this strain did not further metabolize p-nitrophenol (PNP), an intermediate metabolite of MP. No discrepancy in minimum inhibitory concentrations (MICs) to cadmium (Cd), copper (Cu), zinc (Zn), and cobalt (Co) was observed between the engineered X3 strain and its host strain. The inoculated X3 strain accelerated MP degradation in different polluted soil microcosms with 100 mg MP kg(-1) dry soil and/or 5 mg Cd kg(-1) dry soil; MP was completely eliminated within 40 h. However, the presence of Cd in the early stage of remediation slightly delayed MP degradation. The application of X3 strain in Cd-contaminated soil strongly affected the distribution of Cd fractions and immobilized Cd by reducing bioavailable Cd concentrations with lower soluble/exchangeable Cd and organic-bound Cd. The inoculated X3 strain also colonized and proliferated in various contaminated microcosms. Our results suggested that the engineered X3 strain is a potential bioremediation agent showing competitive advantage in complex contaminated environments.
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影响因子：
8.9
作者：
Ardestani, Masoud M.;van Gestel, Cornelis A. M.
通讯作者：
van Gestel, Cornelis A. M.
影响因子：
4.4
作者：
Horne, I;Sutherland, TD;Oakeshott, JG
通讯作者：
Oakeshott, JG
影响因子：
4.4
作者：
Z. Cui;Shun-Peng Li;Guoping Fu
通讯作者：
Z. Cui;Shun-Peng Li;Guoping Fu
DOI：
10.1007/s00128-002-0029-2
发表时间：
2002-06
影响因子：
2.7
作者：
A. Tsatsakis;I. Tsakiris;K. Maxaira;M. Christakis-Hampsas;D. P. Tzanakakis-Nikitovich;N. Niklis
通讯作者：
A. Tsatsakis;I. Tsakiris;K. Maxaira;M. Christakis-Hampsas;D. P. Tzanakakis-Nikitovich;N. Niklis
影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S