Identification of cutaneous fungi and mites in adult atopic dermatitis: analysis by targeted 18S rRNA amplicon sequencing.
Identification of cutaneous fungi and mites in adult atopic dermatitis: analysis by targeted 18S rRNA amplicon sequencing.
复制标题
DOI：
10.1186/s12866-021-02139-9
复制
发表时间：
2021-03-04
期刊：
影响因子：
4.2
通讯作者：
Clausen ML
中科院分区：
文献类型：
--
作者：
Edslev SM;Andersen PS;Agner T;Saunte DML;Ingham AC;Johannesen TB;Clausen ML
特应性皮炎 (AD) 患者的皮肤细菌群落发生了改变，其中含有大量与皮炎相关的金黄色葡萄球菌，这凸显了微生物可能对疾病恶化很重要。尽管有强有力的证据表明细菌皮肤定植与 AD 之间存在关联，但有关 AD 中的真核微生物群落（包括真菌和外寄生虫）的知识非常有限。在这项研究中，我们使用靶向 18S rRNA 扩增子测序比较了成年 AD 患者 (n = 55) 和非 AD 健康对照 (n = 45) 之间的皮肤和鼻腔真核微生物群落。分析基于真核微生物的存在或不存在。 AD 患者和非 AD 个体之间的真核微生物群落的皮肤组成和 α 多样性存在显着差异，AD 皮肤的物种丰富度增加。患者病变和非病变皮肤的α多样性和β多样性相似。外寄生虫毛囊蠕形螨和酵母白地霉在 AD 患者的皮肤上明显更为普遍。最近接受外用皮质类固醇治疗的患者中，皮损皮肤上毛囊毛囊虫的患病率更高。马拉色菌是所有地点最常检测到的属之一，其中球形马拉色菌和限制马拉色菌最为普遍。与非 AD 对照人群相比，限制性限制菌在 AD 患者前鼻孔中的比例较低。 AD 患者和非 AD 个体之间的真核微生物群落存在显着差异，最引人注目的发现是 AD 皮肤上的毛囊 D. 显着过高。毛囊毛囊菌是否会导致 AD 皮肤炎症需要进一步研究。在线版本包含可在 10.1186/s12866-021-02139-9 获取的补充材料。
Atopic dermatitis (AD) patients have an altered skin bacterial community, with an abundance of Staphylococcus aureus associated with flares, highlighting that microbial organisms may be important for disease exacerbation. Despite strong evidence of association between bacterial skin colonisation and AD, very limited knowledge regarding the eukaryotic microbial community, including fungi and ectoparasites, in AD exists. In this study, we compared the skin and nasal eukaryotic microbial community between adult AD patients (n = 55) and non-AD healthy controls (n = 45) using targeted 18S rRNA amplicon sequencing. Analysis was based on the presence or absence of eukaryotic microorganisms. The cutaneous composition of the eukaryotic microbial community and the alpha-diversity differed significantly between AD patients and non-AD individuals, with increased species richness on AD skin. Alpha-diversity and beta-diversity were similar on lesional and non-lesional skin of patients. The ectoparasite Demodex folliculorum and the yeast Geotrichum candidum were significantly more prevalent on the skin of AD patients. The prevalence of D. folliculorum on lesional skin was greater among patients recently treated with topical corticosteroid. Malassezia was one of the most frequently detected genera at all sites, with M. globosa and M. restricta being the most prevalent. M. restricta was under represented in the anterior nares of AD patients as compared to the non-AD control population. Significant differences in the eukaryotic microbial communities were found between AD patients and non-AD individuals, with the most striking finding being the significantly overrepresentation of D. folliculorum on AD skin. Whether D. folliculorum can contribute to skin inflammation in AD needs further investigation. The online version contains supplementary material available at 10.1186/s12866-021-02139-9.
登录
查看更多内容
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.6
作者：
Dolenc-Voljc, M;Pohar, M;Lunder, T
通讯作者：
Lunder, T
DOI：
10.1159/000441545
发表时间：
2016-01-01
期刊：
SKIN BARRIER FUNCTION
影响因子：
--
作者：
Danby, Simon G.
通讯作者：
Danby, Simon G.
影响因子：
2.7
作者：
Elston, Carly A.;Elston, Dirk M.
通讯作者：
Elston, Dirk M.
影响因子：
3.3
作者：
Brandwein, Michael;Fuks, Garold;Meshner, Shiri
通讯作者：
Meshner, Shiri