Functional divergence among silkworm antimicrobial peptide paralogs by the activities of recombinant proteins and the induced expression profiles.
Functional divergence among silkworm antimicrobial peptide paralogs by the activities of recombinant proteins and the induced expression profiles.
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重组蛋白活性和诱导表达谱显示蚕抗菌肽旁系同源物的功能差异
DOI：
10.1371/journal.pone.0018109
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发表时间：
2011-03-29
期刊：
影响因子：
3.7
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Yang W;Cheng T;Ye M;Deng X;Yi H;Huang Y;Tan X;Han D;Wang B;Xiang Z;Cao Y;Xia Q
抗菌肽是一类由多基因家族编码的小分子蛋白质。它们在先天免疫应答中起着至关重要的作用，但关于抗菌肽基因家族功能差异的报道很少。在本研究中，属于天蚕素，moricin和gloverin家族的14个旁系同源物的抗菌肽的重组表达在pET表达系统。抗菌活性试验表明，抗菌肽对10种供试细菌的抗菌活性有显著差异。两个家系的进化速度相对较快，表现出明显的功能分化。格洛弗林家族的四种肽对测试细菌具有相似的抗菌谱和活性。葛洛佛蛋白家族具有相似的抗菌功能和缓慢的进化速度。家蚕蛹感染3种微生物后，通过诱导转录活性，抗菌活性肽编码基因表达水平明显上调。抗菌活性和诱导转录活性的关联分析表明，抗菌活性可能与天蚕素和moricin家族的诱导转录活性呈正相关。这些结果表明，具有代表性的BmcecB6、BmcecD和Bmmora作为主要的效应基因，在各家族中具有广谱、强抗菌活性和高的微生物诱导表达，可能在消除微生物感染中发挥重要作用。
Antimicrobial peptides are small-molecule proteins that are usually encoded by multiple-gene families. They play crucial roles in the innate immune response, but reports on the functional divergence of antimicrobial peptide gene families are rare. In this study, 14 paralogs of antimicrobial peptides belonging to cecropin, moricin and gloverin families were recombinantly expressed in pET expression systems. By antimicrobial activity tests, peptides representing paralogs in the same family of cecropin and moricin families, displayed remarkable differences against 10 tested bacteria. The evolutionary rates were relatively fast in the two families, which presented obvious functional divergence among paralogs of each family. Four peptides of gloverin family had similar antimicrobial spectrum and activity against tested bacteria. The gloverin family showed similar antimicrobial function and slow evolutionary rates. By induced transcriptional activity, genes encoding active antimicrobial peptides were upregulated at obviously different levels when silkworm pupae were infected by three types of microbes. Association analysis of antimicrobial activities and induced transcriptional activities indicated that the antimicrobial activities might be positively correlated with induced transcriptional activities in the cecropin and moricin families. These results suggest that representativeBmcecB6,BmcecDandBmmoras the major effector genes have broad antimicrobial spectrum, strong antimicrobial activity and high microbe-induced expression among each family and maybe play crucial roles in eliminating microbial infection.
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影响因子：
4.8
作者：
HARA, S;YAMAKAWA, M
通讯作者：
YAMAKAWA, M
DOI：
10.1111/j.1432-1033.1994.tb18730.x
发表时间：
1994-04-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
DIMARCQ, JL;HOFFMANN, D;HOFFMANN, JA
通讯作者：
HOFFMANN, JA
影响因子：
--
作者：
Deng XJ;Yang WY;Huang YD;Cao Y;Wen SY;Xia QY;Xu P
通讯作者：
Xu P
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H
影响因子：
10.7
作者：
Duda, TF;Vanhoye, D;Nicolas, P
通讯作者：
Nicolas, P