Stability of monocentric and dicentric ring minichromosomes in Arabidopsis
Stability of monocentric and dicentric ring minichromosomes in Arabidopsis
复制标题
拟南芥单着丝粒和双着丝粒环微染色体的稳定性
DOI：
10.1007/s10577-011-9250-3
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发表时间：
2011
影响因子：
2.6
通讯作者：
M. Murata
中科院分区：
文献类型：
--
作者：
Etsuko Yokota;F. Shibata;K. Nagaki;M. Murata
在转基因拟南芥中鉴定出一个双着丝粒环状微型染色体（miniδ），并将其作为额外染色体添加到野生型中。这条线是相对稳定的，并已保持了几代人，尽管它的环和双中心结构。为了确定miniδ稳定传递的机制，研究了由miniδ衍生的两种新型环状微型染色体（miniδ1和miniδ1-1）的结构和行为。荧光原位杂交分析表明，miniδ1和miniδ一样，是双着丝粒的，而miniδ1-1是单着丝粒的。小δ1和小δ1-1的大小分别为3.8~5.0和1.7Mb。在小δ1上，两个着丝粒的大小相同（约100 μ m）。270 kb），与miniδ1-1（ca. 250 kb）。Miniδ1在有丝分裂和减数分裂过程中相对稳定，miniδ也是如此，而miniδ1-1在有丝分裂过程中不稳定，每个细胞的微型染色体数目变化。这可能是由于单着丝粒小δ1-1上的短着丝粒引起的误分裂所致。通过雌性传递的3个环状微型染色体都很有限（0-3.2%），而通过雄性的传递率相对较高（15.4-27.3%）。没有端粒的环状结构本身似乎并不限制女性传播。
A dicentric ring minichromosome (miniδ) was identified in transgenic Arabidopsis thaliana and added to a wild type as a supernumerary chromosome. This line is relatively stable and has been maintained for generations, notwithstanding its ring and dicentric structure. To determine the mechanism for stable transmission of miniδ, the structure and behavior of two new types of ring minichromosomes (miniδ1 and miniδ1-1) derived from miniδ were investigated. Fluorescence in situ hybridization analysis revealed that miniδ1 is dicentric just like miniδ, whereas miniδ1-1 is monocentric. The estimated sizes of miniδ1 and miniδ1-1 were 3.8~5.0 and 1.7 Mb, respectively. The sizes of the two centromeres on miniδ1 were identical (ca. 270 kb) and similar to that of miniδ1-1 (ca. 250 kb). Miniδ1 was relatively stable during mitosis and meiosis, as is miniδ, whereas miniδ1-1 was unstable during mitosis, and the number of minichromosomes per cell varied. This possibly resulted from misdivision caused by a short centromere on monocentric miniδ1-1. Transmission through the female was quite limited for all three ring minichromosomes (0–3.2%), whereas that through the male was relatively high (15.4–27.3%) compared with that of other supernumerary chromosomes in Arabidopsis. Ring structure without telomeres itself seems not to limit the female transmission.
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影响因子：
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