Towards 3D Bioprinted Spinal Cord Organoids.
Towards 3D Bioprinted Spinal Cord Organoids.
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DOI：
10.3390/ijms23105788
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发表时间：
2022-05-21
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
三维（3D）培养物，即所谓的类器官，已成为疾病建模和治疗创新的一种有吸引力的工具。在这里，我们的目标是确定边界帽神经嵴干细胞（BC）是否可以在基于明胶的3D生物打印生物墨水支架中存活和分化，以建立一种在芯片上制造脊髓类器官的技术。BC先前证明了支持共植入或共培养细胞的存活和分化的能力，并支持兴奋性毒性激发的脊髓切片培养物中的运动神经元存活。我们测试了生物墨水和交联材料的不同组合，分析了BC在支架表面和内部的存活情况，然后测试了人类iPSC衍生的神经细胞（运动神经元前体细胞和星形胶质细胞）是否可以用为BC开发的相同协议打印。我们证明了该方案适用于人类细胞。与打印结构的中央部分相比，神经分化在外周中更为突出，这可能是因为更容易获得培养基中的分化促进因子。这些发现表明，明胶基和酶交联的水凝胶是用于构建多细胞生物打印脊髓类器官的合适生物墨水，但仍需要进一步措施来实现均匀的神经分化。
Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation.
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DOI：
10.1007/s13311-016-0505-8
发表时间：
2017-07
期刊：
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Aggarwal T;Hoeber J;Ivert P;Vasylovska S;Kozlova EN
通讯作者：
Kozlova EN
影响因子：
64.5
作者：
Andersen J;Revah O;Miura Y;Thom N;Amin ND;Kelley KW;Singh M;Chen X;Thete MV;Walczak EM;Vogel H;Fan HC;Paşca SP
通讯作者：
Paşca SP
影响因子：
6
作者：
Garcia-Bennett, Alfonso E.;Kozhevnikova, Mariya;Kozlova, Elena N.
通讯作者：
Kozlova, Elena N.
影响因子：
5.5
作者：
Bose R;Banerjee S;Dunbar GL
通讯作者：
Dunbar GL
影响因子：
8.2
作者：
Grouwels, G.;Vasylovska, S.;Heimberg, H.
通讯作者：
Heimberg, H.