Modeling Neurological Disorders in 3D Organoids Using Human-Derived Pluripotent Stem Cells.
Modeling Neurological Disorders in 3D Organoids Using Human-Derived Pluripotent Stem Cells.
复制标题
利用人源性多能干细胞在三维类器官中建模神经系统疾病。
DOI：
10.3389/fcell.2021.640212
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发表时间：
2021
影响因子：
5.5
通讯作者：
Dunbar GL
中科院分区：
文献类型：
--
作者：
Bose R;Banerjee S;Dunbar GL
神经系统疾病的建模是具有挑战性的，因为它们通常有内源性和外源性原因。脑类器官由三维（3D）自组织脑组织组成，越来越多地被用于模拟大脑发育和疾病的各个方面，如神经元的产生、神经元的迁移和功能网络。这些类器官已被认为是模拟大脑发育特征的重要体外工具，包括神经系统疾病，它可以为这些疾病的分子机制提供见解。在这篇综述中，我们描述了来自诱导多能干细胞（iPSCs）的二维（2D），三维和血脑屏障模型的最新进展，我们讨论了它们在建模疾病方面的优势和局限性，并探索了血管化和功能的脑过程三维模型的发展。本文还综述了脑类器官在模拟主要神经退行性疾病和神经发育障碍方面的应用。
Modeling neurological disorders is challenging because they often have both endogenous and exogenous causes. Brain organoids consist of three-dimensional (3D) self-organizing brain tissue which increasingly is being used to model various aspects of brain development and disorders, such as the generation of neurons, neuronal migration, and functional networks. These organoids have been recognized as important in vitro tools to model developmental features of the brain, including neurological disorders, which can provide insights into the molecular mechanisms involved in those disorders. In this review, we describe recent advances in the generation of two-dimensional (2D), 3D, and blood-brain barrier models that were derived from induced pluripotent stem cells (iPSCs) and we discuss their advantages and limitations in modeling diseases, as well as explore the development of a vascularized and functional 3D model of brain processes. This review also examines the applications of brain organoids for modeling major neurodegenerative diseases and neurodevelopmental disorders.
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影响因子：
46.9
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Cadwell CR;Palasantza A;Jiang X;Berens P;Deng Q;Yilmaz M;Reimer J;Shen S;Bethge M;Tolias KF;Sandberg R;Tolias AS
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Blurton-Jones M