Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers.
Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers.
复制标题
DOI：
10.1021/am100373v
复制
发表时间：
2010-08
影响因子：
9.5
通讯作者：
Advincula, Rigoberto C.
中科院分区：
文献类型：
--
作者：
Baba, Akira;Taranekar, Prasad;Ponnapati, Ramakrishna R.;Knoll, Wolfgang;Advincula, Rigoberto C.
在这项工作中，我们报告了一种电化学表面等离子体共振/波导（EC-SPR/波导）葡萄糖生物传感器，它可以检测导电聚合物/葡萄糖氧化酶（GOx）多层薄膜中的酶反应。为了实现可控的酶电极和波导模式，GOx（带负电荷）固定与水溶性导电N-烷基胺化聚吡咯（带正电荷），使用层层（LbL）静电自组装技术。通过聚吡咯层的电活性和电致变色特性，在加入葡萄糖后，从复合LbL酶电极同时获得电化学和光学信号。在EC-SPR检测中的信号增强是通过监测聚吡咯上的掺杂-脱掺杂事件获得的。真实的时间光信号可以区分酶层介电常数的变化和其他非酶反应事件，如葡萄糖的吸附和溶液折射率的变化。这通过SPR/波导光谱实验中波导的SPR模式（m=0和m=1模式）两者的相关性是可能的。
In this work, we report an electrochemical surface plasmon resonance/waveguide (EC-SPR/waveguide) glucose biosensor, which could detect enzymatic reactions in a conducting polymer/glucose oxidase (GOx) multilayer thin film. In order to achieve a controlled enzyme electrode and waveguide mode, GOx (negatively charged) was immobilized with a water-soluble conducting N-alkylaminated polypyrrole (positively charged) using the layer-by-layer (LbL) electrostatic self-assembly technique. The electrochemical and optical signals were simultaneously obtained from the composite LbL enzyme electrode upon addition of glucose as mediated by the electroactivity and electrochromic property of the polypyrrole layers. The signal enhancement in the EC-SPR detection is obtained by monitoring the doping-dedoping events on the polypyrrole. The real time optical signal could be distinguished between the change in the dielectric constant of the enzyme layer and other non-enzymatic reaction events such as adsorption of glucose and change of refractive index of solution. This was possible by a correlation of both the SPR mode, m=0, and m=1 mode of the waveguide in an SPR/waveguide spectroscopy experiment.
登录
查看更多内容
DOI：
10.1073/pnas.012581399
发表时间：
2002-01-08
影响因子：
11.1
作者：
Wang, DL;Gong, X;Heeger, AJ
通讯作者：
Heeger, AJ
影响因子：
5.4
作者：
Yasuzawa, M;Kunugi, A
通讯作者：
Kunugi, A
影响因子：
3.3
作者：
Baba, A;Advincula, RC;Knoll, W
通讯作者：
Knoll, W
影响因子：
3.9
作者：
Kang, XF;Jin, YD;Dong, SJ
通讯作者：
Dong, SJ
影响因子：
7.4
作者：
UMANA, M;WALLER, J
通讯作者：
WALLER, J