Aptamer in bioanalytical applications.
Aptamer in bioanalytical applications.
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DOI：
10.1021/ac201057w
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发表时间：
2011-06-15
影响因子：
7.4
通讯作者：
Tao, W. Andy
中科院分区：
文献类型：
--
作者：
Iliuk, Anton B.;Hu, Lianghai;Tao, W. Andy
(2009 2010年），适体研究领域不断在科学界产生了大量的兴趣。适体，首先由三个小组独立于1990年报道，1993是人工单链DNA或RNA序列（最近，肽），折叠成二级和三级结构，使它们以极高的特异性结合某些靶点。由于适体对其靶分子（小分子、蛋白质甚至整个细胞）的高特异性亲和力，它被认为类似于化学抗体，解离常数范围从纳摩尔到皮摩尔水平。适体具有许多独特的功能，使它们成为比抗体更有效的选择。首先，适体可以通过体外过程针对合成文库进行筛选，从而可以靶向任何分子（从小的无机离子到完整的细胞），克服了必须使用细胞系或动物的限制，这对于抗体来说是必要的。第二，适体一旦被选择，就可以通过聚合酶链式反应进行随后的扩增，以产生大量的高纯度。第三，核酸适体简单的化学结构使得其易于根据不同的目的进行功能基团的进一步修饰。最后，适体比抗体稳定得多，使其适用于需要苛刻条件的应用（例如，高温或极端pH）。适体的应用仍然非常动态，在生物传感、诊断和治疗领域的探索越来越多（图1中说明了一些基于适体的应用）。近年来，有许多优秀的评论，重点各不相同。在此，作为分析化学中适体的第一次综述，我们试图
(2009À2010), thefield of aptamer research has continuously generated lots of interest in the scientific community. Aptamers, first reported by three groups independently in 1990, 1À3 are the artificial single-stranded DNA or RNA sequences (more recently, peptides) that fold into secondary and tertiary structures making them bind to certain targets with extremely high specificity. Owing to the high specific affinity of an aptamer to its target molecule (small molecules, proteins, and even entire cells), it is thought to resemble chemical antibodies, with the dissociation constants ranging from nanomolar to picomolar level. Aptamers have a number of unique features which make them a more effective choice than antibodies. First, aptamers can be screened via in vitro process against a synthetic library, making it possible to target any molecules (from small inorganic ions to intact cells), overcoming the limit of having to use cell lines or animals, as is necessary for antibodies. Second, aptamers, once selected, can undergo subsequent amplification through polymerase chain reaction to produce a large quantity with high purity. Third, the simple chemical structure of aptamer makes it easily amendable to further modifications with functional groups according to different purposes. Finally, aptamers are much more stable than antibodies, making them suitable in applications requiring harsh conditions (eg, high temperature or extreme pH).The applications of aptamers remain very dynamic, with increasing explorations in the fields of biosensing, diagnostics, and therapeutics (some aptamer-based applications are illustrated in Figure 1). There have been a numbers of excellent reviews in recent years with different emphases. 4À8 Herein, as the first review of aptamers on Analytical Chemistry, we attempt to
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影响因子：
46.9
作者：
Dassie, Justin P.;Liu, Xiu-ying;Thomas, Gregory S.;Whitaker, Ryan M.;Thiel, Kristina W.;Stockdale, Katie R.;Meyerholz, David K.;McCaffrey, Anton P.;McNamara, James O., II;Giangrande, Paloma H.
通讯作者：
Giangrande, Paloma H.
量子点和氧化石墨烯之间的荧光共振能量转移用于传感生物分子
影响因子：
7.4
作者：
Dong, Haifeng;Gao, Wenchao;Ju, Huangxian
通讯作者：
Ju, Huangxian
DOI：
10.1073/pnas.1009331107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Cho, Minseon;Xiao, Yi;Soh, H. Tom
通讯作者：
Soh, H. Tom
影响因子：
16.6
作者：
Cao, Zehui;Tong, Rong;Lu, Yi
通讯作者：
Lu, Yi
用于凝血酶检测的石墨烯荧光共振能量转移适体传感器
影响因子：
7.4
作者：
Chang, Haixin;Tang, Longhua;Li, Jinghong
通讯作者：
Li, Jinghong