Evidence for modulation of osteocalcin containing gamma-carboxyglutamic acid residues synthesis by insulin-like growth factor-I and vitamin K2 in human osteosarcoma cell line MG-63.
Evidence for modulation of osteocalcin containing gamma-carboxyglutamic acid residues synthesis by insulin-like growth factor-I and vitamin K2 in human osteosarcoma cell line MG-63.
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胰岛素样生长因子-I 和维生素 K2 在人骨肉瘤细胞系 MG-63 中调节含有 γ-羧基谷氨酸残基的骨钙素合成的证据。
DOI：
10.1530/eje.0.1380443
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发表时间：
1998
影响因子：
5.8
通讯作者：
Takamura Muraki
中科院分区：
文献类型：
--
作者：
Yoshiki Kudo;Mitsutoshi Iwashita;Yoshihiko Takeda;Takamura Muraki
研究了胰岛素样生长因子-I(IGFI)和维生素K2(2-methyl-3-all-trans-tetraphenyl-1，4-naphtoquinone)对人成骨样细胞(MG-63)合成骨钙素的影响。IGF-I和维生素K2均以浓度依赖方式刺激1，25(OH)2D3诱导的含有GLA的骨钙素的分泌。IGF-I和维生素K2的这种刺激作用是相加的。3-(α-丙酮基-苄基)-4-羟基香豆素(华法林)选择性抑制维生素K2促进的骨钙素分泌。IGF-I的刺激作用可被放线菌酮完全消除；相反，维生素K2在放线菌素存在下的刺激作用仍可观察到。用IGF-I处理MG-63细胞后，骨钙素的mRNA水平增加了约2.2倍(通过逆转录聚合酶链式反应确定)。维生素K2对IGF-I刺激的mRNA水平和基础水平均无影响。IGF-I刺激的含有GLA的骨钙素的分泌可被其结合蛋白之一胰岛素样生长因子结合蛋白-4浓度依赖地抑制。这些结果表明，IGF-I和维生素K2对1.25(OH)2D3诱导的MG-63细胞分泌含有GLA的骨钙素的作用方式不同。
The effect of insulin-like growth factor-I (IGF-I) and 2-methyl-3-all-trans-tetraphenyl-1,4-naphtoquinone (vitamin K2) on the synthesis of osteocalcin containing gamma-carboxyglutamic acid (Gla) residues which is the physiologically relevant form in bone metabolism was studied in cultured human osteoblast-like (MG-63) cells. Both IGF-I and vitamin K2 stimulated 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-induced osteocalcin containing Gla secretion in a concentration-dependent manner. This stimulatory effect of IGF-I and vitamin K2 was additive. Vitamin K2-enhanced osteocalcin containing Gla secretion was selectively suppressed by 3-(alpha-acetonyl-benzyl)-4-hydroxy-coumarin (warfarin). The stimulatory effect of IGF-I was completely abolished by the presence of cycloheximide; in contrast the effect of vitamin K2 was still observed in the presence of cycloheximide. Treatment of MG-63 cells with IGF-I caused an approximately 2.2-fold increase in osteocalcin mRNA levels (determined by reverse transcription-polymerase chain reaction). Vitamin K2 had no effect on either the stimulation of mRNA level by IGF-I or the basal level. IGF-I-stimulated osteocalcin containing Gla secretion was inhibited by one of its binding proteins (insulin-like growth factor binding protein-4) in a concentration-dependent manner. These findings suggest that the modes of action of IGF-I and vitamin K2 on 1.25(OH)2D3-induced osteocalcin containing Gla secretion in MG-63 cells are different.
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影响因子：
4.1
作者：
CANALIS, E;LIAN, JB
通讯作者：
LIAN, JB
影响因子：
20.3
作者：
John I. Jones;D. Clemmons
通讯作者：
John I. Jones;D. Clemmons
影响因子：
4.8
作者：
MCCARTHY, TL;CENTRELLA, M;CANALIS, E
通讯作者：
CANALIS, E
1,25-二羟基维生素 D3 增加骨肉瘤细胞合成维生素 K 依赖性骨蛋白。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Price,PA;Baukol,SA
通讯作者：
Baukol,SA
培养胎鼠颅骨中骨钙素合成的激素调节研究。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lian,JB;Coutts,M;Canalis,E
通讯作者：
Canalis,E