Homeostatic Regulation of Estrus Cycle of Young Female Mice on Western Diet.
Homeostatic Regulation of Estrus Cycle of Young Female Mice on Western Diet.
复制标题
DOI：
10.1210/jendso/bvab010
复制
发表时间：
2021-04-01
影响因子：
4.1
通讯作者：
Burton MD
中科院分区：
文献类型：
--
作者：
Lenert ME;Chaparro MM;Burton MD
生殖障碍的病因与患者的体重增加相关，但生殖、饮食和体重之间的联系在啮齿动物中很难解释。随着儿童肥胖和生殖障碍的增加，研究体重和饮食对青春期女性的影响是关键。先前的研究表明，雌性小鼠对高脂饮食诱导的体重增加具有抵抗力，但其机制尚不清楚。文献还表明，卵巢功能对于抵抗体重增加至关重要，因为卵巢切除术导致体重增加表型与高脂饮食的雄性小鼠相似。然而，尚未评估高脂肪饮食的青春期小鼠中发生的生殖变化。在这里，我们表明，通过孕激素调节发情周期是至关重要的代谢稳态的雌性小鼠在高脂肪饮食。雌性小鼠从4周龄开始接受高脂肪饮食或对照饮食12周。每隔4周，跟踪它们的发情周期并测量空腹血糖。我们发现，高脂饮食4周后，各组之间的体重没有差异，但高脂饮食小鼠的发情前期和发情期时间增加，发情前期血清孕酮增加。这些结果表明，完整的女性调节其发情周期，以响应高脂肪饮食作为体内平衡调节体重的机制，保护它们免受代谢异常。了解这种保护背后的机制可能会产生治疗青春期女性患者生殖障碍的治疗机会。
The etiology of reproductive disorders correlates with weight gain in patients, but the link between reproduction, diet, and weight has been difficult to translate in rodents. As rates of childhood obesity and reproductive disorders increase, the need to study the effects of weight and diet on adolescent females is key. Previous studies show that female mice are resistant to high-fat diet–induced weight gain, but the mechanisms are unclear. Literature also suggests that ovarian function is essential to resistance in weight gain, as an ovariectomy leads to a weight-gaining phenotype similar to male mice on a high-fat diet. However, reproductive changes that occur in adolescent mice on high-fat diet have not been assessed. Here, we show that regulation of the estrus cycle via progesterone is critical to metabolic homeostasis in female mice on a high-fat diet. Female mice were put on high-fat diet or control diet for 12 weeks starting at 4 weeks of age. Every 4 weeks, their estrus cycle was tracked and fasting glucose was measured. We found that after 4 weeks on high-fat diet, there was no difference in weight between groups, but an increase in time spent in proestrus and estrus in mice on high-fat diet and an increase in serum progesterone during proestrus. These results show that intact females modulate their estrus cycle in response to a high-fat diet as a mechanism of homeostatic regulation of body weight, protecting them from metabolic abnormalities. Understanding the mechanisms behind this protection may yield therapeutic opportunities for treatment of reproductive disorders in adolescent female patients.
登录
查看更多内容
DOI：
10.1073/pnas.1601825113
发表时间：
2016-04-12
影响因子：
11.1
作者：
Kubota, Kaiyu;Cui, Wei;Soares, Michael J.
通讯作者：
Soares, Michael J.
影响因子：
5.2
作者：
Palmisano BT;Stafford JM;Pendergast JS
通讯作者：
Pendergast JS
影响因子：
4.8
作者：
Martin, Bronwen;Pearson, Michele;Mattson, Mark P.
通讯作者：
Mattson, Mark P.
影响因子：
7.3
作者：
Lainez NM;Jonak CR;Nair MG;Ethell IM;Wilson EH;Carson MJ;Coss D
通讯作者：
Coss D
DOI：
10.3791/4389
发表时间：
2012-09-15
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
McLean AC;Valenzuela N;Fai S;Bennett SA
通讯作者：
Bennett SA