Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.).
Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.).
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DOI：
10.1371/journal.pone.0057500
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Röder MS
中科院分区：
文献类型：
--
作者：
Kollers S;Rodemann B;Ling J;Korzun V;Ebmeyer E;Argillier O;Hinze M;Plieske J;Kulosa D;Ganal MW;Röder MS
共358个最近的欧洲冬小麦品种和14个春小麦品种进行了评价赤霉病（FHB）引起的禾谷镰刀菌和镰刀菌culmorum在四个不同的环境中的抗性。基于赤霉病发病率（I型抗性）×赤霉病严重度（II型抗性）的赤霉病评分显示了品种的广泛表型变异，BLUE（最佳线性无偏估计）值范围为0.07 - 33.67。用732个微卫星标记进行基因分型，得到782个位点，其中620个位点位于ITMI图谱上。得到的平均标记距离为6.8 cM，允许采用混合模型进行全基因组关联作图。虽然没有发现明确的人口结构，亲属矩阵用于分层。共检测到794个SSR位点与环境特异性FHB评分或BLUE值之间的显著（− log 10（p）-值≥3.0）关联，其中包括323个SSR等位基因。对于FHB发病率和FHB严重程度，分别检测到861和877个个体标记-性状关联（MTA）。在大多数情况下，这两个性状与FHB评分相关。在三个或三个以上的环境中检测到的一致的协会被发现在所有染色体上，除了染色体6 B，并与最高数量的MTA染色体5 B。一个品种内有利和不利等位基因的数量与各自的FHB评分的依赖性表明有利和不利等位基因的加性效应，即具有更有利或更少不利等位基因的基因型倾向于表现出对FHB的更大抗性。对矮秆基因Rht-D1的特异性标记的评估产生了强烈的影响。研究结果为设计全基因组抗赤霉病育种策略提供了前提。
A total of 358 recent European winter wheat varieties plus 14 spring wheat varieties were evaluated for resistance to Fusarium head blight (FHB) caused by Fusarium graminearum and Fusarium culmorum in four separate environments. The FHB scores based on FHB incidence (Type I resistance)×FHB severity (Type II resistance) indicated a wide phenotypic variation of the varieties with BLUE (best linear unbiased estimation) values ranging from 0.07 to 33.67. Genotyping with 732 microsatellite markers resulted in 782 loci of which 620 were placed on the ITMI map. The resulting average marker distance of 6.8 cM allowed genome wide association mapping employing a mixed model. Though no clear population structure was discovered, a kinship matrix was used for stratification. A total of 794 significant (−log10(p)-value≥3.0) associations between SSR-loci and environment-specific FHB scores or BLUE values were detected, which included 323 SSR alleles. For FHB incidence and FHB severity a total of 861 and 877 individual marker-trait associations (MTA) were detected, respectively. Associations for both traits co-located with FHB score in most cases. Consistent associations detected in three or more environments were found on all chromosomes except chromosome 6B, and with the highest number of MTA on chromosome 5B. The dependence of the number of favourable and unfavourable alleles within a variety to the respective FHB scores indicated an additive effect of favourable and unfavourable alleles, i.e. genotypes with more favourable or less unfavourable alleles tended to show greater resistance to FHB. Assessment of a marker specific for the dwarfing gene Rht-D1 resulted in strong effects. The results provide a prerequisite for designing genome wide breeding strategies for FHB resistance.
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影响因子：
3.3
作者：
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通讯作者：
Sorrells, ME
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