In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels.
In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels.
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DOI：
10.1039/c7bm00110j
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发表时间：
2017-09-26
影响因子：
6.6
通讯作者：
Annabi N
中科院分区：
文献类型：
--
作者：
Noshadi I;Hong S;Sullivan KE;Shirzaei Sani E;Portillo-Lara R;Tamayol A;Shin SR;Gao AE;Stoppel WL;Black LD III;Khademhosseini A;Annabi N
可光交联材料经常用于构建用于组织工程的软仿生水凝胶。尽管紫外线 (UV) 光通常用于光交联此类材料，但其使用与多种生物安全问题相关，例如 DNA 损伤、组织加速老化和癌症。在这里，我们报告了一种用于心肌再生的可注射可见光交联明胶基水凝胶。机械表征表明，通过改变前体配方中共引发剂和共聚单体的浓度，可以在 5-56 kPa 范围内调整工程水凝胶的压缩模量。此外，平均孔径（26-103 μm）和溶胀率（7-13%）也可以通过改变水凝胶配方来调节。体外研究表明，可见光交联的 GelMA 水凝胶支持原代心肌细胞 (CM) 的生长和功能。此外，该工程材料在体内被证明具有生物相容性，并且可以在动物模型中心肌梗塞后成功递送至心脏，以促进组织愈合。所开发的可见光交联水凝胶可用于修复心肌等多种软组织，并可用于治疗心血管疾病，增强治疗功能。
Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although, ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive moduli of the engineered hydrogels could be tuned in the range of 5–56 kPa, by changing the concentrations of the co-initiator and co-monomer in the precursor formulation. In addition, the average pore sizes (26–103 μm) and swelling ratio (7–13%) were also shown to be tunable by varying the hydrogel formulation. In vitro studies showed that visible light crosslinked GelMA hydrogels supported the growth and function of primary cardiomyocytes (CMs). In addition, the engineered materials were shown to be biocompatible in vivo, and could be successfully delivered to the heart after myocardial infarction in an animal model to promote tissue healing. The developed visible light crosslinked hydrogel could be used for the repair of various soft tissues such as the myocardium, and for the treatment of cardiovascular diseases with enhanced therapeutic functionality.
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DOI：
10.1002/adma.201503255
发表时间：
2016-01-06
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
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作者：
Annabi N;Shin SR;Tamayol A;Miscuglio M;Bakooshli MA;Assmann A;Mostafalu P;Sun JY;Mithieux S;Cheung L;Tang XS;Weiss AS;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
4.1
作者：
Akins, Robert E., Jr.;Rockwood, Danielle;Pizarro, Christian
通讯作者：
Pizarro, Christian
影响因子：
9.7
作者：
Hao, Yiting;Shih, Han;Munoz, Zachary;Kemp, Arika;Lin, Chien-Chi
通讯作者：
Lin, Chien-Chi
影响因子：
6.2
作者：
Fu, Amy;Gwon, Kihak;Kornfield, Julia A.
通讯作者：
Kornfield, Julia A.
DOI：
10.1007/bf01081407
发表时间：
1969-01-01
期刊：
Histochemical Journal
影响因子：
--
作者：
GOLDSTEIN D J
通讯作者：
GOLDSTEIN D J