RNA structure analysis of alphacoronavirus terminal genome regions.
RNA structure analysis of alphacoronavirus terminal genome regions.
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DOI：
10.1016/j.virusres.2014.10.001
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发表时间：
2014-12-19
期刊：
影响因子：
5
通讯作者：
Ziebuhr J
中科院分区：
文献类型：
--
作者：
Madhugiri R;Fricke M;Marz M;Ziebuhr J
回顾当前对冠状病毒复制必不可少的CIS作用RNA元素的知识。
鉴定字母内病毒末端基因组区域中RNA结构元件。
讨论基因组和贝塔曲纳病毒中基因组顺序作用RNA元件的基因组内和内部保守性的讨论。
冠状病毒基因组复制是由多种蛋白质复合物介导的，该蛋白质复合物由十多个病毒编码和几个细胞蛋白组成，病毒蛋白与CIS作用RNA的相互作用与5' - 末端基因组中的CIS作用RNA元素相互作用。美国基因组复制已广泛在Betacoronaviruse中的特征，在较小程度上，我们在这里回顾了当前对冠状病毒的理解。我们，但也越过总体而言，在α-和betacoronavirus的基因组中确定的5'末端RNA结构元素确定的属边界与Coronavirinae的广泛使用的复制蛋白分类一致
Review of current knowledge of cis-acting RNA elements essential to coronavirus replication. Identification of RNA structural elements in alphacoronavirus terminal genome regions. Discussion of intra- and intergeneric conservation of genomic cis-acting RNA elements in alpha- and betacoronaviruses. Coronavirus genome replication is mediated by a multi-subunit protein complex that is comprised of more than a dozen virally encoded and several cellular proteins. Interactions of the viral replicase complex with cis-acting RNA elements located in the 5′ and 3′-terminal genome regions ensure the specific replication of viral RNA. Over the past years, boundaries and structures of cis-acting RNA elements required for coronavirus genome replication have been extensively characterized in betacoronaviruses and, to a lesser extent, other coronavirus genera. Here, we review our current understanding of coronavirus cis-acting elements located in the terminal genome regions and use a combination of bioinformatic and RNA structure probing studies to identify and characterize putative cis-acting RNA elements in alphacoronaviruses. The study suggests significant RNA structure conservation among members of the genus Alphacoronavirus but also across genus boundaries. Overall, the conservation pattern identified for 5′ and 3′-terminal RNA structural elements in the genomes of alpha- and betacoronaviruses is in agreement with the widely used replicase polyprotein-based classification of the Coronavirinae, suggesting co-evolution of the coronavirus replication machinery with cognate cis-acting RNA elements.
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冠状病毒主蛋白酶的结构揭示了甲over依蛋白酶折叠与额外的α-螺旋结构域的组合。
影响因子：
11.4
作者：
Anand, Kanchan;Palm, Gottfried J;Mesters, Jeroen R;Siddell, Stuart G;Ziebuhr, John;Hilgenfeld, Rolf
通讯作者：
Hilgenfeld, Rolf
影响因子：
3.7
作者：
Brockway SM;Denison MR
通讯作者：
Denison MR
影响因子：
56.9
作者：
Anand, K;Ziebuhr, J;Hilgenfeld, R
通讯作者：
Hilgenfeld, R
影响因子：
3.7
作者：
Chen, Shih-Cheng;Olsthoorn, Rene C. L.
通讯作者：
Olsthoorn, Rene C. L.
影响因子：
64.5
作者：
Brierley I;Digard P;Inglis SC
通讯作者：
Inglis SC