Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China.
Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China.
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中国大宗木本油料作物油茶树种的遗传关系和低多样性
DOI：
10.3389/fpls.2022.996731
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发表时间：
2022
影响因子：
5.6
通讯作者：
Zheng, Daojun
中科院分区：
文献类型：
--
作者：
Qi, Huasha;Sun, Xiuxiu;Yan, Wuping;Ye, Hang;Chen, Jiali;Yu, Jing;Jun, Dai;Wang, Chunmei;Xia, Tengfei;Chen, Xuan;Li, Dongliang;Zheng, Daojun
关键词：
油茶是世界四大木本油料作物之一，具有很高的生态、经济和药用价值。然而，在茶油茶派的分类和合并上存在很大的差异。油茶种类繁多，给油茶资源的创新利用和生产带来了困难。利用ISSR、SRAP和叶绿体序列标记对油茶组18个居群进行了分子标记分析。探索油菜属植物的系统发育关系和遗传多样性。结果表明，它们的遗传多样性较低，平均H和π分别为0.16和0.00140。群体间遗传分化程度较高，ISSR和SRAP标记Fst为0.38，最高Nm为1.77，cpDNA标记Fst为0.65，Nm最低为0.27。海南岛的华南华南、华南华南和海南岛的种群组成了一个单一的类群，显示出最接近的亲缘关系，并支持将它们作为同一种，统一命名为华南华南，并将海南岛上的资源归类为华南华南。海南岛的油茶资源应归为核果茶属的一个特殊的生态类型或变种。然而，基于cpDNA标记的结构分析表明，桂花种群的遗传成分形成了一个独立的纯合子簇，因此桂花应该是组中的一个新种。油菜属。油茶(C.oleifera var.)单子植物和油菜种群聚为两个不同的支系，油菜变种。单子植物居群形成了一个独立的类群，占其遗传组成的99.00%以上，而油茶居群包含多个不同的聚类组分，表明油茶变异。单种植物与油茶明显不同，应该被认为是独立的种。单倍型分析表明，供试种群的单倍型没有迅速扩大，油茶、甜瓜和桂花的单倍型是由核桃的单倍型进化而来的。本研究结果支持油茶亚属的系统发育分类，对油茶育种和生产具有重要意义。
Tea-oil Camellia is one of the four woody oil crops in the world and has high ecological, economic and medicinal values. However, there are great differences in the classification and merging of tea-oil Camellia Sect. Oleifera species, which brings difficulties to the innovative utilization and production of tea-oil Camellia resources. Here, ISSR, SRAP and chloroplast sequence markers were analyzed in 18 populations of tea-oil Camellia Sect. Oleifera species to explore their phylogenetic relationships and genetic diversity. The results showed that their genetic diversity were low, with mean H and π values of 0.16 and 0.00140, respectively. There was high among-population genetic differentiation, with ISSR and SRAP markers showing an Fst of 0.38 and a high Nm of 1.77 and cpDNA markers showing an Fst of 0.65 and a low Nm of 0.27. The C. gauchowensis, C. vietnamensis and Hainan Island populations formed a single group, showing the closest relationships, and supported being the same species for them with the unifying name C. drupifera and classifying the resources on Hainan Island as C. drupifera. The tea-oil Camellia resources of Hainan Island should be classified as a special ecological type or variety of C. drupifera. However, cpDNA marker-based STRUCTURE analysis showed that the genetic components of the C. osmantha population formed an independent, homozygous cluster; hence, C. osmantha should be a new species in Sect. Oleifera. The C. oleifera var. monosperma and C. oleifera populations clustered into two distinct clades, and the C. oleifera var. monosperma populations formed an independent cluster, accounting for more than 99.00% of its genetic composition; however, the C. oleifera populations contained multiple different cluster components, indicating that C. oleifera var. monosperma significantly differs from C. oleifera and should be considered the independent species C. meiocarpa. Haplotype analysis revealed no rapid expansion in the tested populations, and the haplotypes of C. oleifera, C. meiocarpa and C. osmantha evolved from those of C. drupifera. Our results support the phylogenetic classification of Camellia subgenera, which is highly significant for breeding and production in tea-oil Camellia.
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影响因子：
2.4
作者：
Lu, Xuelin;Chen, Hailing;Tang, Shaoqing
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5.6
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