Role for cER and Mmr1p in anchorage of mitochondria at sites of polarized surface growth in budding yeast.
Role for cER and Mmr1p in anchorage of mitochondria at sites of polarized surface growth in budding yeast.
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CER和MMR1P在萌芽酵母中极化表面生长部位的线粒体锚固中的作用。
DOI：
10.1016/j.cub.2011.10.019
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发表时间：
2011-12-06
期刊：
影响因子：
--
通讯作者：
Pon LA
中科院分区：
文献类型：
--
作者：
Swayne TC;Zhou C;Boldogh IR;Charalel JK;McFaline-Figueroa JR;Thoms S;Yang C;Leung G;McInnes J;Erdmann R;Pon LA
线粒体积聚在神经元和免疫突触和酵母芽尖端，并在磷脂生物合成、钙稳态和线粒体裂变过程中与内质网相关。我们发现线粒体与芽尖质膜下的皮质内质网（cER）相关，并证实了YPT11的缺失，抑制了芽尖上cER的积累，也抑制了芽尖上线粒体的锚定。延时成像显示，线粒体固定在芽尖的特定位置。Mmr1p是DSL1系栓蛋白家族的一员，定位于芽尖下线粒体和cER片相对表面的点状结构，并与分离的线粒体和内质网一起恢复。MMR1的缺失损害了线粒体的芽尖锚定，但不影响线粒体速度或cER分布。磷酸酶PTC1的缺失导致Mmr1p磷酸化增加，Mmr1p定位错误，Mmr1p与线粒体和内质网的关联缺陷，以及线粒体芽尖锚定缺陷。这些发现表明，Mmr1p作为酵母芽尖线粒体锚定到cER片的介质，参与线粒体遗传，而Ptc1p调节Mmr1p的磷酸化、定位和功能。
Mitochondria accumulate at neuronal and immunological synapses and yeast bud tips, and associate with the ER during phospholipid biosynthesis, calcium homeostasis and mitochondrial fission. We show that mitochondria are associated with cortical ER (cER) sheets underlying the plasma membrane in the bud tip, and confirmed that a deletion in YPT11, which inhibits cER accumulation in the bud tip, also inhibits bud tip anchorage of mitochondria. Time-lapse imaging reveals that mitochondria are anchored at specific sites in the bud tip. Mmr1p, a member of the DSL1 family of tethering proteins, localizes to punctate structures on opposing surfaces of mitochondria and cER sheets underlying the bud tip, and is recovered with isolated mitochondria and ER. Deletion of MMR1 impairs bud tip anchorage of mitochondria, without affecting mitochondrial velocity or cER distribution. Deletion of the phosphatase PTC1 results in increased Mmr1p phosphorylation, mislocalization of Mmr1p, defects in association of Mmr1p with mitochondria and ER, and defects in bud tip anchorage of mitochondria. These findings indicate that Mmr1p contributes to mitochondrial inheritance as a mediator of anchorage of mitochondria to cER sheets in the yeast bud tip, and that Ptc1p regulates Mmr1p phosphorylation, localization and function.
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DOI：
10.1083/jcb.2.4.193
发表时间：
1956-07-25
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
PALAY SL
通讯作者：
PALAY SL
影响因子：
3.3
作者：
Roeder, AD;Hermann, GJ;Shaw, JM
通讯作者：
Shaw, JM
DOI：
10.1073/pnas.2033246100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Shepard, KA;Gerber, AP;Vale, RD
通讯作者：
Vale, RD
影响因子：
7.8
作者：
McFaline-Figueroa JR;Vevea J;Swayne TC;Zhou C;Liu C;Leung G;Boldogh IR;Pon LA
通讯作者：
Pon LA
影响因子：
3.3
作者：
Boldogh, IR;Ramcharan, SL;Pon, LA
通讯作者：
Pon, LA