Longer and faster sperm exhibit better fertilization success in Japanese quail.
Longer and faster sperm exhibit better fertilization success in Japanese quail.
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DOI：
10.1016/j.psj.2021.01.003
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发表时间：
2021-04
期刊：
影响因子：
4.4
通讯作者：
Sasanami T
中科院分区：
文献类型：
--
作者：
Matsuzaki M;Hirohashi N;Tsudzuki M;Haqani MI;Maeda T;Mizushima S;Sasanami T
在鸟类中，位于子宫阴道交界处的精子储存小管（SST）被认为是精子选择的场所。然而，人们对精子选择的确切机制知之甚少。在这里，我们研究了在对 2 名雄性精子混合物进行人工授精所产生的竞争情况下，精子进入 SST 以及随后的受精成功率。我们采用了 2 种鹌鹑品系，一种是野生型，一种是优势黑色 (DB) 型，因为这样可以通过羽毛颜色轻松评估亲子关系。我们发现，当来自 2 个雄性品系的精子数量相似的精子被人工授精给雌性时，胚胎的亲子鉴定偏向 DB 雄性。我们使用 2 种不同荧光染料的新型精子染色方法表明，DB 偏向受精是因为 DB 精子进入 SST 的能力更好。此外，我们发现 DB 精子具有更长的鞭毛和中段。这些特性可能使精子在高粘度介质中游动得更快，这可能是与女性生殖道管腔相似的环境。我们的结果表明，精子竞争是为了在 SST 中赢得一席之地，而用自己的精子填充 SST 是雄性日本鹌鹑获得更好受精成功的关键步骤。
In birds, sperm storage tubules (SST) located in the utero-vaginal junction are thought to be a site of sperm selection; however, the exact mechanism of sperm selection is poorly understood. Here, we investigated sperm entry into the SST and subsequent fertilization success under a competitive situation created by artificial insemination of a sperm mixture obtained from 2 males. We employed 2 quail strains, a wild-type and a dominant black (DB) type, as this allows easy assessment of paternity by feather coloration. We found paternity of embryos was biased toward DB males when a sperm mix with similar sperm numbers from the 2 males strains was artificially inseminated into females. Our novel sperm staining method with 2 different fluorescent dyes showed that the DB-biased fertilization was because of the better ability of DB sperm to enter the SST. Moreover, we found that DB sperm had a longer flagellum and midpiece. These characteristics probably allow sperm to swim faster in a high viscosity medium, which may be a similar environment to the lumen of the female reproductive tract. Our results indicated that sperm competition occurs to win a place in the SST and that filling the SST with their own spermatozoa is a critical step to achieve better fertilization success for the male Japanese quail.
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DOI：
10.1098/rspb.2000.1577
发表时间：
2001-04-07
影响因子：
4.7
作者：
Evans, JP;Magurran, AE
通讯作者：
Magurran, AE
影响因子：
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作者：
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影响因子：
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影响因子：
16.8
作者：
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通讯作者：
Pizzari T
影响因子：
3.8
作者：
King, LA;Brillard, JP;Donoghue, AA
通讯作者：
Donoghue, AA