Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco.
Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco.
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DOI：
10.1093/jxb/err229
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发表时间：
2011-10
影响因子：
6.9
通讯作者：
Liu JH
中科院分区：
文献类型：
--
作者：
Huang XS;Luo T;Fu XZ;Fan QJ;Liu JH
丝裂原活化蛋白激酶（MAPK）级联反应在与植物发育和胁迫反应相关的多种信号通路中起着关键作用。本研究以枳（Poncirus trifoliata（L.）Raf报道。PtrMAPK含有11个高度保守的激酶结构域和一个磷酸化基序（TEY），定位于转化洋葱表皮细胞的细胞核中。PtrMAPK转录水平增加脱水和寒冷，但不受盐。PtrMAPK在烟草中的转基因过量表达赋予脱水和耐旱性。与野生型相比，转基因植株表现出更好的水分状况，更少的活性氧（ROS）产生，更高水平的抗氧化酶活性和代谢产物。有趣的是，抗氧化酶抑制剂降低了转基因植物的抗胁迫能力。此外，在正常或干旱条件下，PtrMAPK的过表达增强了ROS相关和胁迫响应基因的表达。两者合计，这些数据表明，PtrMAPK作为一个积极的调节剂在脱水/干旱胁迫反应，通过调节活性氧稳态通过激活细胞的抗氧化系统或调节转录水平的各种压力相关的基因。
The mitogen-activated protein kinase (MAPK) cascade plays pivotal roles in diverse signalling pathways related to plant development and stress responses. In this study, the cloning and functional characterization of a group-I MAPK gene, PtrMAPK, in Poncirus trifoliata (L.) Raf are reported. PtrMAPK contains 11 highly conserved kinase domains and a phosphorylation motif (TEY), and is localized in the nucleus of transformed onion epidermal cells. The PtrMAPK transcript level was increased by dehydration and cold, but was unaffected by salt. Transgenic overexpression of PtrMAPK in tobacco confers dehydration and drought tolerance. The transgenic plants exhibited better water status, less reactive oxygen species (ROS) generation, and higher levels of antioxidant enzyme activity and metabolites than the wild type. Interestingly, the stress tolerance capacity of the transgenic plants was compromised by inhibitors of antioxidant enzymes. In addition, overexpression of PtrMAPK enhanced the expression of ROS-related and stress-responsive genes under normal or drought conditions. Taken together, these data demonstrate that PtrMAPK acts as a positive regulator in dehydration/drought stress responses by either regulating ROS homeostasis through activation of the cellular antioxidant systems or modulating transcriptional levels of a variety of stress-associated genes.
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影响因子：
4.2
作者：
Aswath, CR;Kim, SH;Kim, DH
通讯作者：
Kim, DH
影响因子：
7.2
作者：
Ichimura, K;Mizoguchi, T;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
2.7
作者：
Pogány, M;Koehl, J;Barna, B
通讯作者：
Barna, B
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
DOI：
10.1073/pnas.96.26.15354
发表时间：
1999-12-21
影响因子：
11.1
作者：
Qin, XQ;Zeevaart, JAD
通讯作者：
Zeevaart, JAD