Recombinant expression, purification, and characterization of an acyl-CoA binding protein from Aspergillus oryzae
Recombinant expression, purification, and characterization of an acyl-CoA binding protein from Aspergillus oryzae
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米曲霉酰基辅酶 A 结合蛋白的重组表达、纯化和表征
DOI：
10.1007/s10529-015-2003-1
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发表时间：
2015-12
影响因子：
2.7
通讯作者：
Bin Zeng
中科院分区：
文献类型：
--
作者：
Qing Hao;Xiaoguang Liu;Guozhong Zhao;Lu Jiang;Ming Li;Bin Zeng
目的：研究工业真菌曲霉（Aspergillus）脂代谢调节酰基辅酶A结合蛋白（ACBP）的生物化学特性。目的：克隆麦芽糖结合蛋白（AoACBP）基因，并在大肠杆菌中表达。MBP-AoACBP蛋白通过直链淀粉树脂层析柱纯化。SDS-PAGE显示MBP-AoACBP的分子量约为82 kDa。微量热泳结合实验表明，重组AoACBP对棕榈酰辅酶A的亲和力（Kd = 80 nM）远大于对肉豆蔻酰辅酶A的亲和力（Kd = 510 nM），表明AoACBP对长链酰基辅酶A具有较强的亲和力。米。
Objectives:To characterize biochemically the lipid metabolism-regulating acyl-CoA binding protein (ACBP) from the industrially-important fungus Aspergillus oryzae.Results:A full-length cDNA encoding a candidate ACBP from A. oryzae (AoACBP) was cloned and expressed in Escherichia coli as a maltose-binding protein (MBP) fusion protein. The MBP-AoACBP protein was purified by an amylose resin chromatography column. SDS-PAGE showed that MBP-AoACBP has an estimated molecular weight of 82 kDa. Microscale thermophoresis binding assay showed that the recombinant AoACBP displayed much greater affinity for palmitoyl-CoA (K d = 80 nM) than for myristoyl-CoA (K d = 510 nM), thus demonstrating the preference of AoACBP for long-chain acyl-CoA.Conclusion:The data support the identification of AoACBP as a long-chain ACBP in A. oryzae.
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影响因子：
4.1
作者：
Burton, M;Rose, TM;Knudsen, J
通讯作者：
Knudsen, J
影响因子：
64.8
作者：
Parker, Joanne L.;Newstead, Simon
通讯作者：
Newstead, Simon
影响因子：
13.6
作者：
S. Xiao;M. Chye
通讯作者：
S. Xiao;M. Chye
DOI：
10.1093/dnares/dsn020
发表时间：
2008-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Machida M;Yamada O;Gomi K
通讯作者：
Gomi K
影响因子：
1.5
作者：
B. Zeng;Xiaomin Cai;G. Zhu
通讯作者：
B. Zeng;Xiaomin Cai;G. Zhu