Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future.
Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future.
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DOI：
10.1093/dnares/dsn020
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发表时间：
2008-08
期刊：
影响因子：
--
通讯作者：
Gomi K
中科院分区：
文献类型：
--
作者：
Machida M;Yamada O;Gomi K
在没有微生物概念的时代，我们的祖先凭经验建立了生产各种发酵食品的方法：味噌（豆腐调味料）和酱油（酱料），这两种食品都被广泛使用，是日本烹饪的基本要素，清酒是一种在各种仪式场合饮用的神奇酒精饮料，是典型的例子。一种丝状真菌，曲霉菌，是生产所有这些传统食品的关键生物，其固态培养（SSC）已被证实是发酵过程中至关重要的分泌型水解酶的高生产率的秘密。事实上，我们的基因组比较和转录组分析揭示了SSC中原料有效降解的机制：仅在A.在A.在SSC中，但在液体培养中不诱导烟曲霉。此外，在传统的酱油发酵过程中经验性地采用的降温过程已被发现对保持A的强表达是重要的。水稻特异性胞外水解酶。A.值得注意的是，它已成功地应用于生物降解塑料的有效降解。在A.米。基因组分析与传统知识和技术相结合将继续是未来探索A.米。
At a time when the notion of microorganisms did not exist, our ancestors empirically established methods for the production of various fermentation foods: miso (bean curd seasoning) and shoyu (soy sauce), both of which have been widely used and are essential for Japanese cooking, and sake, a magical alcoholic drink consumed at a variety of ritual occasions, are typical examples. A filamentous fungus, Aspergillus oryzae, is the key organism in the production of all these traditional foods, and its solid-state cultivation (SSC) has been confirmed to be the secret for the high productivity of secretory hydrolases vital for the fermentation process. Indeed, our genome comparison and transcriptome analysis uncovered mechanisms for effective degradation of raw materials in SSC: the extracellular hydrolase genes that have been found only in the A. oryzae genome but not in A. fumigatus are highly induced during SSC but not in liquid cultivation. Also, the temperature reduction process empirically adopted in the traditional soy-sauce fermentation processes has been found to be important to keep strong expression of the A. oryzae-specific extracellular hydrolases. One of the prominent potentials of A. oryzae is that it has been successfully applied to effective degradation of biodegradable plastic. Both cutinase, responsible for the degradation of plastic, and hydrophobin, which recruits cutinase on the hydrophobic surface to enhance degradation, have been discovered in A. oryzae. Genomic analysis in concert with traditional knowledge and technology will continue to be powerful tools in the future exploration of A. oryzae.
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影响因子：
3.5
作者：
Cramer, Robert A., Jr.;Stajich, Jason E.;Perfect, John R.
通讯作者：
Perfect, John R.
DOI：
10.1073/pnas.71.7.2848
发表时间：
1974-01-01
影响因子：
11.1
作者：
KIMURA, M;OHTA, T
通讯作者：
OHTA, T
影响因子：
2.5
作者：
Akao, T;Gomi, K;Akita, O
通讯作者：
Akita, O
影响因子：
2.8
作者：
Klich, MA
通讯作者：
Klich, MA
影响因子：
1.2
作者：
GOMI, K;TANAKA, A;TAKAHASHI, K
通讯作者：
TAKAHASHI, K