Simultaneous determination of multiple amino acids in plasma in critical illness by high performance liquid chromatography with ultraviolet and fluorescence detection.
Simultaneous determination of multiple amino acids in plasma in critical illness by high performance liquid chromatography with ultraviolet and fluorescence detection.
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DOI：
10.1016/j.jchromb.2013.09.016
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发表时间：
2013-12-01
影响因子：
3
通讯作者：
Anstey, Nicholas M.
中科院分区：
文献类型：
--
作者：
Wang, Hao;McNeil, Yvette R.;Yeo, Tsin W.;Anstey, Nicholas M.
人们越来越认识到，宿主对危重疾病的反应包括多种氨基酸途径的紊乱，包括对代谢、免疫、内皮和神经功能至关重要的氨基酸 (AA)。为了表征这些血浆氨基酸的浓度变化，我们报告了一种使用 HPLC 并同时进行 UV 和荧光 (FL) 检测来定量小体积血浆 (50 μL) 中 AA 的方法的开发和验证。使用 6-氨基喹啉基-N-羟基琥珀酰亚胺基氨基甲酸酯 (AQC) 进行蛋白质沉淀和柱前衍生化，然后进行反相 HPLC 分离。使用正亮氨酸作为内标建立校准曲线。选择 33 种（包括 20 种蛋白原）AA 作为标准品，并对健康对照者和严重恶性疟疾患者血浆中相应的浓度进行定量。该方法能够检测精氨酸代谢、芳香氨基酸途径、甲硫氨酸转硫和转甲基化途径以及其他代谢途径中的扰动。
There is increasing recognition that the host response to critical illness includes derangement of multiple amino acid pathways, including amino acids (AAs) central to metabolism and immune, endothelial and neurological function. To characterise concentration changes of these plasma amino acid we report the development and validation of a method for the quantification of AAs in small volumes of plasma (50 μL) using HPLC with simultaneous UV and fluorescence (FL) detection. Protein precipitation and pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) is followed by reversed phase HPLC separation. Calibration curves were built with norleucine as an internal standard. Thirty three (including the 20 proteinogenic) AAs, were selected as standards and their corresponding concentrations in the plasma of healthy human controls and patients with severe falciparum malaria were quantified. This method enables the detection of perturbations in arginine metabolism, aromatic amino acid pathways, methionine transsulfuration and transmethylation pathways and other metabolic pathways.
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影响因子：
6.4
作者：
Yeo, Tsin W.;Lampah, Daniel A.;Anstey, Nicholas M.
通讯作者：
Anstey, Nicholas M.
影响因子：
3
作者：
Eckstein, James A.;Ammerman, Gina M.;Ackermann, Bradley L.
通讯作者：
Ackermann, Bradley L.
DOI：
10.5713/ajas.2009.90156
发表时间：
2009-12-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Li, Junyou;Piao, Chunxiang;Manabe, Noboru
通讯作者：
Manabe, Noboru
影响因子：
168.9
作者：
Lopansri, BK;Anstey, NM;Granger, DL
通讯作者：
Granger, DL
DOI：
10.1186/cc8055
发表时间：
2009
期刊：
Critical care (London, England)
影响因子：
--
作者：
Davis JS;Yeo TW;Thomas JH;McMillan M;Darcy CJ;McNeil YR;Cheng AC;Celermajer DS;Stephens DP;Anstey NM
通讯作者：
Anstey NM