Biocompatibility implications of polypyrrole synthesis techniques.
Biocompatibility implications of polypyrrole synthesis techniques.
复制标题
聚吡咯合成技术的生物相容性影响。
DOI：
10.1088/1748-6041/3/3/034124
复制
发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Fonner JM;Forciniti L;Nguyen H;Byrne JD;Kou YF;Syeda-Nawaz J;Schmidt CE
聚吡咯（PPy）是一种固有的导电聚合物，在神经系统的生物医学应用中显示出巨大的前景。然而，为了有效地使用聚吡啶作为生物材料植入物，了解和可复制地控制聚合物的电学性质、物理形貌和表面化学是很重要的。尽管有很多关于聚吡啶在各种应用中的应用的研究发表，但还没有系统的研究将聚吡啶的合成方法与聚吡啶的基本聚合物特性（如亲水性、表面粗糙度）以及这些特性对细胞的生物效应联系起来。电化学合成的聚吡啶薄膜的特性取决于合成参数，如掺杂剂、衬底和厚度等参数。在这些研究中，我们使用了三种掺杂剂(氯化物（Cl）， tosylate （ToS)，聚苯乙烯磺酸盐（PSS）），两种衬底（金和铟锡氧化玻璃），以及一系列厚度，来测量和比较生物医学上重要的表面粗糙度，接触角，电导率，掺杂剂稳定性和细胞粘附性（使用PC-12细胞和Schwann细胞）。正如预测的那样，我们发现粗糙度的差异很大，取决于所使用的掺杂剂和薄膜的厚度，而衬底的选择几乎没有影响。通过接触角测量，发现PSS产生了最亲水的材料，这很可能是因为长PSS链暴露在PPy表面上的自由电荷。掺杂tos的PPy薄膜的导电性比掺杂Cl或pss的薄膜高10倍。x射线光电子能谱研究用于评估在水和磷酸盐缓冲盐水中储存超过14天的PPy薄膜的掺杂浓度，并在同一时间段内进行电导研究以测量电稳定性。PSS被证明是最稳定的掺杂剂，尽管所有薄膜的电导率和掺杂浓度都有明显的衰减。细胞粘附研究表明，细胞结果依赖于膜厚度和掺杂剂的选择。正如这些实验所证明的那样，不同合成参数的优缺点是设计生物医学植入物时必须考虑的关键设计因素。这些研究的结果应该为研究导电聚合物，特别是PPy的研究人员在合成参数、聚合物性质和生物相容性之间的关系方面提供实用的见解。
Polypyrrole (PPy) is an inherently conducting polymer that has shown great promise for biomedical applications within the nervous system. However, to effectively use PPy as a biomaterial implant, it is important to understand and reproducibly control the electrical properties, physical topography, and surface chemistry of the polymer. Although there is much research published on the use of PPy in various applications, there is no systematic study linking the methodologies used for PPy synthesis to PPy’s basic polymeric properties (e.g., hydrophilicity, surface roughness), and to the biological effects these properties have on cells. Electrochemically synthesized PPy films differ greatly in their characteristics depending on synthesis parameters such as dopant, substrate, and thickness, among other parameters. In these studies, we have used three dopants (chloride (Cl), tosylate (ToS), polystyrene sulfonate (PSS)), two substrates (gold and indium tin oxide-coated glass), and a range of thicknesses, to measure and compare the biomedically-important characteristics of surface roughness, contact angle, conductivity, dopant stability, and cell adhesion (using PC-12 cells and Schwann cells). As predicted, we discovered large differences in roughness depending on the dopant used and the thickness of the film, while substrate choice had little effect. From contact angle measurements, PSS was found to yield the most hydrophilic material, most likely because of free charges from the long PSS chains exposed on the surface of the PPy. ToS-doped PPy films were tenfold more conductive than Cl- or PSS-doped films. X-ray photoelectron spectroscopy studies were used to evaluate dopant concentrations of PPy films stored in water and phosphate buffered saline over 14 days, and conductance studies over the same timeframe measured electrical stability. PSS proved to be the most stable dopant, though all films experienced significant decay in conductivity and dopant concentration. Cell adhesion studies demonstrated the dependence of cell outcome on film thickness and dopant choice. The strengths and weaknesses of different synthesis parameters, as demonstrated by these experiments, are critical design factors that must be leveraged when designing biomedical implants. The results of these studies should provide practical insight to researchers working with conducting polymers, and particularly PPy, on the relationships between synthesis parameters, polymeric properties, and biological compatibility.
登录
查看更多内容
影响因子：
5.7
作者：
Bentley, David;O'Connor, Timothy P.
通讯作者：
O'Connor, Timothy P.
影响因子：
4.4
作者：
KUHN, HH;CHILD, AD;KIMBRELL, WC
通讯作者：
KIMBRELL, WC
影响因子：
--
作者：
Miller, C;Jeftinija, S;Mallapragada, S
通讯作者：
Mallapragada, S
影响因子：
14
作者：
Cui, XY;Wiler, J;Martin, DC
通讯作者：
Martin, DC
影响因子：
1.1
作者：
BOLTO, BA;MCNEILL, R;WEISS, DE
通讯作者：
WEISS, DE