The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity.
The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity.
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初始接种比例调节细菌共培养相互作用和代谢能力
DOI：
10.1038/s41396-020-00751-7
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Sørensen SJ
中科院分区：
文献类型：
--
作者：
Gao CH;Cao H;Cai P;Sørensen SJ
共培养是微生物生态学研究中一个重要的模型系统。初始接种比作为一个关键的试验参数，对共培养体系的结果有着至关重要的影响。然而，这种效应从未在多个生态位条件下进行过研究。在本研究中，我们在不同碳源下建立了两种模式细菌的简单共培养体系，并研究了初始接种比为1:1000 ~ 1000:1对群落结构、功能和细菌相互作用的影响。我们发现，不同初始接种比的共培养物的最终比例在大约五分之六的碳源上存在差异，这表明最终比例高度依赖于初始接种比，而细菌的碳源偏好并不能预测共培养物的最终比例。此外，我们发现初始比例可以调节共培养物的代谢能力，只有初始比例为1:1和1000:1的共培养物在14种特定碳源上获得较高的代谢能力。其根本原因可能是初始比值改变了物种相互作用的模式。综上所述，我们发现初始比例可以诱导共培养的涌现特性。这些发现表明，初始比例不仅会影响共培养实验的可重复性，而且会影响我们对一般微生物生态学的理解。
Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology.
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影响因子：
11
作者：
Lee, Kai Wei Kelvin;Yam, Joey Kuok Hoong;Rice, Scott A.
通讯作者：
Rice, Scott A.
影响因子：
11
作者：
Liu, Wenzheng;Jacquiod, Samuel;Sorensen, Soren J.
通讯作者：
Sorensen, Soren J.
影响因子：
11
作者：
Goyal, Akshit;Dubinkina, Veronika;Maslov, Sergei
通讯作者：
Maslov, Sergei
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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影响因子：
11
作者：
Beliaev, Alexander S.;Romine, Margie F.;Konopka, Allan
通讯作者：
Konopka, Allan