In vivo magnetic resonance imaging of sodium and diffusion in rat glioma at 21.1 T.
In vivo magnetic resonance imaging of sodium and diffusion in rat glioma at 21.1 T.
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DOI：
10.1002/mrm.23077
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发表时间：
2012-04
影响因子：
3.3
通讯作者：
Levenson, Cathy W.
中科院分区：
文献类型：
--
作者：
Schepkin, Victor D.;Bejarano, Fabian Calixto;Morgan, Thomas;Gower-Winter, Shannon;Ozambela, Manuel, Jr.;Levenson, Cathy W.
使用21.1T下钠MRI的高级灵敏度，在6-8天内评价了颅内大鼠9 L胶质瘤的钠和弥散MRI。胶质瘤倍增时间为2.4-2.6天。使用0.15 ms的超短回波时间检测胶质瘤钠信号。像素为0.125 µL的高分辨率3D钠MRI可以最大限度地减少通常与低强度信号MRI相关的部分容积效应。通过术前检测，评价了对1，3-双（2-氯乙基）-1-亚硝基脲（BCNU）具有不同抗性的两个独立的9 L细胞亚克隆的肿瘤钠和扩散MRI。在体内，植入后，与非抗性9 L细胞（78±3 mM）相比，抗性9 L细胞产生了钠浓度显著降低的肿瘤（57±3 mM）。相应的扩散差异较小，但也有统计学意义。在肿瘤进展期间，在两种细胞类型中观察到胶质瘤钠浓度增加，相对于正常脑，增加率为2.4- 5.8%/天。此时肿瘤扩散没有显著变化，表明胶质瘤细胞结构没有改变。因此，肿瘤进展过程中钠的变化反映了细胞内钠浓度的增加和代谢应激的增加。这些实验还证明了钠MRI反映肿瘤细胞抗性的增强的灵敏度。
Sodium and diffusion MRI in intracranial rat 9L gliomas were evaluated over 6–8 days using the advanced sensitivity of sodium MRI at 21.1T. Glioma doubling time was 2.4–2.6 days. Glioma sodium signal was detected using the ultra-short echo time of 0.15 ms. The high resolution 3D sodium MRI with pixels of 0.125 µL allowed for minimizing a partial volume effect often relevant to the MRI of low intensity signals. Tumor sodium and diffusion MRI were evaluated for two separate sub-clones of 9L cells with different resistance to 1,3 bis(2-chloroethyl)-1-nitrosurea (BCNU) detected by pre-surgery assays. In vivo, after implantation, resistant 9L cells created tumors with significantly reduced sodium concentrations (57±3 mM) compared to non-resistant 9L cells (78±3 mM). The corresponding differences in diffusion were less, but also statistically significant. During tumor progression, an increase of glioma sodium concentration was observed in both cell types with a rate of 2.4–5.8 %/day relative to normal brain. Tumor diffusion was not significantly changed at this time, indicative of no alterations in glioma cellularity. Thus, changes in sodium during tumor progression reflect increasing intracellular sodium concentration and mounting metabolic stress. These experiments also demonstrate an enhanced sensitivity of sodium MRI to reflect tumor cell resistance.
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影响因子：
3.9
作者：
IZUMU, I;MINEURA, K;KOWADA, M
通讯作者：
KOWADA, M
影响因子：
3.8
作者：
Ouwerkerk, Ronald;Jacobs, Michael A.;Bottomley, Paul A.
通讯作者：
Bottomley, Paul A.
影响因子：
3.3
作者：
Christensen, JD;Barrere, BJ;Thulborn, KR
通讯作者：
Thulborn, KR
影响因子：
4.8
作者：
Babsky, AM;Hekmatyar, SK;Bansal, N
通讯作者：
Bansal, N
影响因子：
2.3
作者：
Chenevert, Thomas L.;Ross, Brian D.
通讯作者：
Ross, Brian D.