Facilitation of rice stripe virus accumulation in the insect vector by Himetobi P virus VP1.
Facilitation of rice stripe virus accumulation in the insect vector by Himetobi P virus VP1.
复制标题
DOI：
10.3390/v7031492
复制
发表时间：
2015-03-23
期刊：
影响因子：
--
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li S;Ge S;Wang X;Sun L;Liu Z;Zhou Y
褐飞虱是水稻条纹叶枯病病毒的主要传播媒介，在东亚地区引起严重的水稻条纹叶枯病。为了表征病毒-载体相互作用，使用基于GAL 4的酵母双杂交系统，以RSV核糖核蛋白（RNP）作为诱饵筛选SBPH cDNA文库。鉴定了RSV-RNP与Himeplani P病毒（HiPV，一种昆虫小核糖核酸样病毒）VP 1蛋白之间的相互作用。进一步研究了HiPV和RSV在SBPH中的相互关系，结果表明，VP 1表达量高的个体，RSV的滴度普遍较高。在VP 1基因被RNA沉默抑制后，RSV在昆虫体内的积累显著减少，而SBPH的病毒获得能力不受影响，这表明HiPV VP 1可能促进RSV在SBPH中的积累。
The small brown planthopper (SBPH) is the main vector for rice stripe virus (RSV), which causes serious rice stripe disease in East Asia. To characterize the virus-vector interactions, the SBPH cDNA library was screened with RSV ribonucleoprotein (RNP) as bait using a GAL4-based yeast two-hybrid system. The interaction between RSV-RNP and the Himetobi P virus (HiPV, an insect picorna-like virus) VP1 protein was identified. The relationships between HiPV and RSV in SBPH were further investigated, and the results showed that the titer of RSV was commonly higher in single insect that exhibited more VP1 expression. After the VP1 gene was repressed by RNA silencing, the accumulation of RSV decreased significantly in the insect, whereas the virus acquisition ability of SBPH was unaffected, which suggests that HiPV VP1 potentially facilitates the accumulation of RSV in SBPH.
登录
查看更多内容
植物病毒的跨卵巢传播是由其昆虫载体的卵黄蛋白原介导的
影响因子：
6.7
作者：
Huo Y;Liu W;Zhang F;Chen X;Li L;Liu Q;Zhou Y;Wei T;Fang R;Wang X
通讯作者：
Wang X
影响因子：
11.4
作者：
Spahn, CMT;Gomez-Lorenzo, MG;Frank, J
通讯作者：
Frank, J
影响因子：
3.8
作者：
VANDENHEUVEL, JFJM;VERBEEK, M;VANDERWILK, F
通讯作者：
VANDERWILK, F
影响因子：
2.7
作者：
Nakashima, N;Sasaki, J;Toriyama, S
通讯作者：
Toriyama, S
影响因子：
9.2
作者：
Horman, S;Browne, GJ;Rider, MH
通讯作者：
Rider, MH