Advances in approaches to study cell-type specific cortical circuits throughout development.
Advances in approaches to study cell-type specific cortical circuits throughout development.
复制标题
DOI：
10.3389/fncel.2022.1031389
复制
发表时间：
2022
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
作者：
新皮层和海马体中的神经元是多样的，并根据它们的类型形成突触连接。最近的工作提高了我们对神经元细胞类型以及如何将其作为实验目标的理解。这对于研究皮层回路结构至关重要，因为相对于神经元亚型的多样性，目前已建立的细胞类型特异性回路图案的目录很小。其中一些图案遍布大脑皮层，这表明它们是基本计算所必需的典型电路。然而，回路组织在大脑中的定型程度或在皮层区域的变化程度仍然不清楚。皮层回路也是可塑的，它们的组织在每个发育阶段都在进化。因此，实验访问与时间控制的神经元亚型是必不可少的研究皮层的结构和功能。在这篇简短的综述中，我们重点介绍了针对特定神经元亚型的几项最新进展，并研究了它们在整个发育过程中的突触连接和生理学。我们强调的方法，联合收割机多种技术，提供成功的应用程序的例子，并描述潜在的未来应用的新工具。
Neurons in the neocortex and hippocampus are diverse and form synaptic connections that depend on their type. Recent work has improved our understanding of neuronal cell-types and how to target them for experiments. This is crucial for investigating cortical circuit architecture, as the current catalog of established cell-type specific circuit motifs is small relative to the diversity of neuronal subtypes. Some of these motifs are found throughout the cortex, suggesting they are canonical circuits necessary for basic computations. However, the extent to which circuit organization is stereotyped across the brain or varies by cortical region remains unclear. Cortical circuits are also plastic, and their organization evolves throughout each developmental stage. Thus, experimental access to neuronal subtypes with temporal control is essential for studying cortical structure and function. In this mini review, we highlight several recent advances to target specific neuronal subtypes and study their synaptic connectivity and physiology throughout development. We emphasize approaches that combine multiple techniques, provide examples of successful applications, and describe potential future applications of novel tools.
登录
查看更多内容
小鼠大脑皮层 GABA 神经元组合靶向的策略和工具
影响因子：
16.2
作者：
He M;Tucciarone J;Lee S;Nigro MJ;Kim Y;Levine JM;Kelly SM;Krugikov I;Wu P;Chen Y;Gong L;Hou Y;Osten P;Rudy B;Huang ZJ
通讯作者：
Huang ZJ
影响因子：
8.8
作者：
Cheung V;Chung P;Bjorni M;Shvareva VA;Lopez YC;Feinberg EH
通讯作者：
Feinberg EH
影响因子：
3
作者：
Adam, Yoav
通讯作者：
Adam, Yoav
影响因子：
--
作者：
Fenno, Lief E;Mattis, Joanna;Deisseroth, Karl
通讯作者：
Deisseroth, Karl
影响因子：
16.2
作者：
Graybuck LT;Daigle TL;Sedeño-Cortés AE;Walker M;Kalmbach B;Lenz GH;Morin E;Nguyen TN;Garren E;Bendrick JL;Kim TK;Zhou T;Mortrud M;Yao S;Siverts LA;Larsen R;Gore BB;Szelenyi ER;Trader C;Balaram P;van Velthoven CTJ;Chiang M;Mich JK;Dee N;Goldy J;Cetin AH;Smith K;Way SW;Esposito L;Yao Z;Gradinaru V;Sunkin SM;Lein E;Levi BP;Ting JT;Zeng H;Tasic B
通讯作者：
Tasic B