Peptide nanovesicles formed by the self-assembly of branched amphiphilic peptides.
Peptide nanovesicles formed by the self-assembly of branched amphiphilic peptides.
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DOI：
10.1371/journal.pone.0045374
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tomich JM
中科院分区：
文献类型：
--
作者：
Gudlur S;Sukthankar P;Gao J;Avila LA;Hiromasa Y;Chen J;Iwamoto T;Tomich JM
基于肽的包装系统作为更安全的药物递送系统显示出巨大的潜力。它们克服了与基于脂质或病毒的递送系统、维斯稳定性、特异性、炎症、抗原性和调节能力相关的问题。在这里，我们描述了一组15和23个残基的分支，两亲性肽，模仿磷酸甘油酯的分子结构。这些肽经历超分子自组装并形成具有50-200 nm直径的溶剂填充的双层界定的球体，如TEM、STEM和DLS所证实。虽然弱疏水力驱动和维持脂双层组装，但这些全肽结构可能通过疏水相互作用和氢键来稳定，并在低微摩尔浓度和较高温度下保持完整。缺少分支点的线性肽没有显示出自组装特性。我们已经观察到这些肽囊泡可以在其内部捕获荧光染料分子，并被培养物中生长的N/N 1003 A兔透镜上皮细胞摄取。因此，这些组件是可以克服当前包装系统的一些关键限制的潜在药物递送系统。
Peptide-based packaging systems show great potential as safer drug delivery systems. They overcome problems associated with lipid-based or viral delivery systems, vis-a-vis stability, specificity, inflammation, antigenicity, and tune-ability. Here, we describe a set of 15 & 23-residue branched, amphiphilic peptides that mimic phosphoglycerides in molecular architecture. These peptides undergo supramolecular self-assembly and form solvent-filled, bilayer delimited spheres with 50–200 nm diameters as confirmed by TEM, STEM and DLS. Whereas weak hydrophobic forces drive and sustain lipid bilayer assemblies, these all-peptide structures are stabilized potentially by both hydrophobic interactions and hydrogen bonds and remain intact at low micromolar concentrations and higher temperatures. A linear peptide lacking the branch point showed no self-assembly properties. We have observed that these peptide vesicles can trap fluorescent dye molecules within their interior and are taken up by N/N 1003A rabbit lens epithelial cells grown in culture. These assemblies are thus potential drug delivery systems that can overcome some of the key limitations of the current packaging systems.
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影响因子：
3.4
作者：
Koynova, Rumiana;Tarahovsky, Yury S.;MacDonald, Robert C.
通讯作者：
MacDonald, Robert C.
影响因子：
6.2
作者：
GRUEN, LC
通讯作者：
GRUEN, LC
影响因子：
3
作者：
BROOKS, BR;BRUCCOLERI, RE;KARPLUS, M
通讯作者：
KARPLUS, M
影响因子：
41.2
作者：
Bellomo, EG;Wyrsta, MD;Deming, TJ
通讯作者：
Deming, TJ
DOI：
10.1073/pnas.1237107100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Hoshi, M;Sato, M;Sato, K
通讯作者：
Sato, K