Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence.
Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence.
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DOI：
10.1093/nar/gkx953
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发表时间：
2018-01-09
影响因子：
14.9
通讯作者：
Helmann JD
中科院分区：
文献类型：
--
作者：
Gaballa A;Guariglia-Oropeza V;Dürr F;Butcher BG;Chen AY;Chandrangsu P;Helmann JD
细菌适应压力的能力取决于特定基因组的条件表达。枯草芽孢杆菌编码七种细胞质外功能（ECF）σ（σ）因子，其调节在引发细胞包膜应激的条件下对存活重要的功能。其中四个已被详细研究：σM，σW，σX和σV。这四个σ因子识别重叠的启动子组，尽管决定这种重叠识别的序列还不完全清楚。启动子选择性的主要作用被归因于核心-10和-35启动子元件。在这里，我们证明了一个均聚的T-tract基序，接近-35元素，功能与核心启动子序列相结合，以确定ECF σ因子的选择性。该基序对于通过σV激活启动子是最关键的，并且有助于通过σM、σX和σW激活。我们提出，这个基序，这是一个功能的推导启动子共识的一个子集ECF σ因子从许多物种，赋予固有的DNA曲率影响启动子活性。因此，ECF σ因子中该区域的差异效应提供了调节子重叠的性质和程度的机制。
The ability of bacteria to adapt to stress depends on the conditional expression of specific sets of genes. Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma (σ) factors that regulate functions important for survival under conditions eliciting cell envelope stress. Of these, four have been studied in detail: σM, σW, σX and σV. These four σ factors recognize overlapping sets of promoters, although the sequences that determine this overlapping recognition are incompletely understood. A major role in promoter selectivity has been ascribed to the core −10 and −35 promoter elements. Here, we demonstrate that a homopolymeric T-tract motif, proximal to the −35 element, functions in combination with the core promoter sequences to determine selectivity for ECF sigma factors. This motif is most critical for promoter activation by σV, and contributes variably to activation by σM, σX and σW. We propose that this motif, which is a feature of the deduced promoter consensus for a subset of ECF σ factors from many species, imparts intrinsic DNA curvature to influence promoter activity. The differential effect of this region among ECF σ factors thereby provides a mechanism to modulate the nature and extent of regulon overlap.
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