Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size

Regulatory change at Physalis Organ Size 1 correlates to natural variation in tomatillo reproductive organ size
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酸浆器官大小 1 的监管变化与番茄生殖器官大小的自然变化相关

DOI:
10.1038/ncomms5271
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发表时间:
2014-07
影响因子:
16.6
通讯作者:
Chaoying He
Chaoying He
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Jing Li;Jing Zhao;Zhichao Li;Chaoying He

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西红柿(Physalis Philadelphica)生殖器官大小变异的遗传基础尚不清楚。在这里,我们报道了Phsalis器官大小1(Pos1)基因的表达水平与花、浆果和种子等繁殖器官的大小变化呈正相关。与野生型相比,Pos1基因敲除导致花朵和浆果更小,细胞更小。相反,POS1的过度表达会增加器官的大小,而不会增加细胞数量。来自大、中、小番茄群体的pos1等位基因的第一个内含子分别含有一个、两个和三个37bp的重复序列。此外,我们的结果表明,POS1等位基因第一内含子中重复序列的复制变异导致了该基因的差异表达。因此,番茄生殖器官大小的共同变异可以归因于POS1的新的调控变异。
The genetic basis of size variation in the reproductive organs of tomatillo (Physalis philadelphica) is unknown. Here we report that the expression levels of the gene Physalis Organ Size 1 (POS1) are positively associated with size variation in P. philadelphica reproductive organs such flowers, berries and seeds. POS1 knockdown results in smaller flowers and berries with smaller cells as compared with their wild-type counterparts. Conversely, POS1 overexpression promotes organ size without increasing the cell number. The first introns of the POS1 alleles from the large, intermediate and small tomatillo groups contain one, two and three 37-bp repeats, respectively. Furthermore, our results show that copy variation of repeats in the first intron of POS1 alleles results in differential expression of this gene. Thus, co-variation in tomatillo reproductive organ sizes can be attributed to the novel regulatory variation in POS1.
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