Inoculation density and nutrient level determine the formation of mushroom-shaped structures in Pseudomonas aeruginosa biofilms.
Inoculation density and nutrient level determine the formation of mushroom-shaped structures in Pseudomonas aeruginosa biofilms.
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DOI：
10.1038/srep32097
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发表时间：
2016-09-09
影响因子：
4.6
通讯作者：
Meyer-Hermann M
中科院分区：
文献类型：
--
作者：
Ghanbari A;Dehghany J;Schwebs T;Müsken M;Häussler S;Meyer-Hermann M
铜绿假单胞菌常在免疫功能低下患者和囊性纤维化患者的肺中定植。它通过形成生物膜对许多抗生素产生耐药性，这使得它很难被消灭。铜绿假单胞菌生物膜在某些情况下形成蘑菇状结构。细菌的运动和环境影响最终的蘑菇形态。本研究为细菌动力学和影响细菌生物膜形状的相互作用提供了一个基于agent的模型。模型中的细胞运动依赖于最近发表的实验数据。我们的模拟显示了不动细胞形成的集落。运动细胞通过营养物趋化性从单个菌落中逃逸，因此不会形成蘑菇形状。非运动菌落的高数量密度导致运动细胞迁移到菌落顶部，形成蘑菇状结构。该模型提出可以通过接种细菌时的参数来预测蘑菇形状结构的形成。根据营养水平和秸秆的初始数量密度，蘑菇形状的结构只能在有限的条件下形成。这开启了利用环境因素早期操纵细菌菌落空间格局形成的可能性。
Pseudomonas aeruginosa often colonises immunocompromised patients and the lungs of cystic fibrosis patients. It exhibits resistance to many antibiotics by forming biofilms, which makes it hard to eliminate. P. aeruginosa biofilms form mushroom-shaped structures under certain circumstances. Bacterial motility and the environment affect the eventual mushroom morphology. This study provides an agent-based model for the bacterial dynamics and interactions influencing bacterial biofilm shape. Cell motility in the model relies on recently published experimental data. Our simulations show colony formation by immotile cells. Motile cells escape from a single colony by nutrient chemotaxis and hence no mushroom shape develops. A high number density of non-motile colonies leads to migration of motile cells onto the top of the colonies and formation of mushroom-shaped structures. This model proposes that the formation of mushroom-shaped structures can be predicted by parameters at the time of bacteria inoculation. Depending on nutrient levels and the initial number density of stalks, mushroom-shaped structures only form in a restricted regime. This opens the possibility of early manipulation of spatial pattern formation in bacterial colonies, using environmental factors.
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影响因子：
1.9
作者：
Dockery, J;Klapper, I
通讯作者：
Klapper, I
影响因子：
3.6
作者：
Boles, BR;Thoendel, M;Singh, PK
通讯作者：
Singh, PK
DOI：
10.1073/pnas.1315521110
发表时间：
2014-01-07
影响因子：
11.1
作者：
Kempes, Christopher P.;Okegbe, Chinweike;Dietrich, Lars E. P.
通讯作者：
Dietrich, Lars E. P.
影响因子：
5.1
作者：
Barken, Kim B.;Pamp, Suenje J.;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim
影响因子：
5.4
作者：
Boyle KE;Heilmann S;van Ditmarsch D;Xavier JB
通讯作者：
Xavier JB