Combined fluorescence and X-Ray tomography for quantitative in vivo detection of fluorophore.
Combined fluorescence and X-Ray tomography for quantitative in vivo detection of fluorophore.
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DOI：
10.1177/153303461000900105
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Gulsen G
中科院分区：
文献类型：
--
作者：
Barber WC;Lin Y;Nalcioglu O;Iwanczyk JS;Hartsough NE;Gulsen G
介绍了一种新型双模临床前成像仪的初步结果，该成像仪结合了非接触式荧光断层扫描（FT）和x射线计算机断层扫描（CT），用于临床前功能和体内解剖成像。CT的解剖数据为FT重建提供了先验信息，以创建重叠的功能和解剖图像，准确定位和量化荧光团的分布。含有吲哚菁绿（ICG）内含物的幻影，具有不同背景，包括不同浓度的碘，用于CT对比，已通过双模态FT/CT系统成像。来自衰减图的解剖信息和来自吸收和散射图的光学形态学信息被用作FT重建的先验信息。虽然可以在没有先验信息的情况下定位ICG包裹体，但恢复的ICG浓度有75%的误差。当利用先验信息时，ICG浓度的恢复误差仅为15%。发展在感兴趣的解剖区域准确量化荧光团浓度的能力可能为体内小动物成像提供一个强大的工具。
Initial results from a novel dual modality preclinical imager which combines non-contact fluorescence tomography (FT) and x-ray computed tomography (CT) for preclinical functional and anatomical in vivo imaging are presented. The anatomical data from CT provides a priori information to the FT reconstruction to create overlaid functional and anatomical images with accurate localization and quantification of fluorophore distribution. Phantoms with inclusions containing Indocyanine-Green (ICG), and with heterogeneous backgrounds including iodine in compartments at different concentrations for CT contrast, have been imaged with the dual modality FT/CT system. Anatomical information from attenuation maps and optical morphological information from absorption and scattering maps are used as a priori information in the FT reconstruction. Although ICG inclusions can be located without the a priori information, the recovered ICG concentration shows 75% error. When the a priori information is utilized, the ICG concentration can be recovered with only 15% error. Developing the ability to accurately quantify fluorophore concentration in anatomical regions of interest may provide a powerful tool for in vivo small animal imaging.
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影响因子：
--
作者：
Grimm, J.;Kircher, M. F.;Weissleder, R.
通讯作者：
Weissleder, R.
DOI：
10.1016/j.nima.2004.03.065
发表时间：
2004-06-01
影响因子：
1.4
作者：
Hasegawa, BH;Barber, WC;Seo, Y
通讯作者：
Seo, Y
影响因子：
46.9
作者：
Ahrens, ET;Flores, R;Morel, PA
通讯作者：
Morel, PA
影响因子：
1.9
作者：
Lin Y;Yan H;Nalcioglu O;Gulsen G
通讯作者：
Gulsen G
影响因子：
2.8
作者：
Gulsen, Gultekin;Birgul, Ozlem;Nalcioglu, Orhan
通讯作者：
Nalcioglu, Orhan