Oral delivery mediated RNA interference of a carboxylesterase gene results in reduced resistance to organophosphorus insecticides in the cotton Aphid, Aphis gossypii Glover.
Oral delivery mediated RNA interference of a carboxylesterase gene results in reduced resistance to organophosphorus insecticides in the cotton Aphid, Aphis gossypii Glover.
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DOI：
10.1371/journal.pone.0102823
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gao XW
中科院分区：
文献类型：
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作者：
Gong YH;Yu XR;Shang QL;Shi XY;Gao XW
RNA干扰（RNAi）是检测单个基因功能的有效工具。羧酸酯酶（carboxylesterase, CarE, EC 3.1.1.1）在许多昆虫物种的外源化合物代谢中起着重要作用。本实验室前期研究发现，棉蚜（Aphis gossypii (Glover)）半翅目：蚜科（Aphis gossypii，半翅目：蚜科）抗氧乐果品系和抗马拉硫磷品系成虫中CarE的表达均上调，提示CarE可能参与有机磷（OP）杀虫剂抗性。因此，有必要通过功能分析（RNAi）来研究CarE在棉蚜对OPs抗性中的作用。摄入dsRNA-CarE后，棉蚜抗氧乐果个体中CarE的表达被显著抑制。dsRNA-CarE浓度为100 ng/µL时，饲喂后72 h敲除效率最高（33%）。被敲除的蚜虫的CarE活性与相应的CarE表达显著降低一致。对照蚜虫体内的CarE活性集中在650 ~ 900 mOD/ /min之间，饲喂dsrna -CarE的蚜虫体内的CarE活性集中在500 ~ 800 mOD/ /min之间。体外抑制实验也表明，与对照蚜虫相比，CarE敲除蚜虫的总CarE活性显著降低。饲喂dsRNA-CarE的蚜虫生物测定结果表明，抑制CarE表达增加了抗性蚜虫个体对乐果的敏感性。本研究结果不仅表明通过人工饲料摄取dsRNA可用于棉蚜的功能基因组研究，而且表明CarE可被认为是棉蚜对有机磷杀虫剂（OPs）抗性的主要靶点。此外，我们的研究结果表明，CarE可能是OPs抗性蚜虫控制的有利靶点，通过植物rnai介导的昆虫CarE表达沉默可以获得抗虫转基因植物。
RNA interference (RNAi) is an effective tool to examine the function of individual genes. Carboxylesterases (CarE, EC 3.1.1.1) are known to play significant roles in the metabolism of xenobiotic compounds in many insect species. Previous studies in our laboratory found that CarE expression was up-regulated in Aphis gossypii (Glover) (Hemiptera: Aphididae) adults of both omethoate and malathion resistant strains, indicating the potential involvement of CarE in organophosphorus (OP) insecticide resistance. Functional analysis (RNAi) is therefore warranted to investigate the role of CarE in A. gossypii to OPs resistance. CarE expression in omethoate resistant individuals of Aphis gossypii was dramatically suppressed following ingestion of dsRNA-CarE. The highest knockdown efficiency (33%) was observed at 72 h after feeding when dsRNA-CarE concentration was 100 ng/µL. The CarE activities from the CarE knockdown aphids were consistent with the correspondingly significant reduction in CarE expression. The CarE activity in the individuals of control aphids was concentrated in the range of 650–900 mOD/per/min, while in the individuals of dsRNA-CarE-fed aphids, the CarE activity was concentrated in the range of 500–800 mOD/per/min. In vitro inhibition experiments also demonstrated that total CarE activity in the CarE knockdown aphids decreased significantly as compared to control aphids. Bioassay results of aphids fed dsRNA-CarE indicated that suppression of CarE expression increased susceptibility to omethoate in individuals of the resistant aphid strains. The results of this study not only suggest that ingestion of dsRNA through artificial diet could be exploited for functional genomic studies in cotton aphids, but also indicate that CarE can be considered as a major target of organophosphorus insecticide (OPs) resistance in A. gossypii. Further, our results suggest that the CarE would be a propitious target for OPs resistant aphid control, and insect-resistant transgenic plants may be obtained through plant RNAi-mediated silencing of insect CarE expression.
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RNAi在豌豆蚜虫acyrthosiphon pisum中敲除基因。
影响因子：
3.5
作者：
Jaubert-Possamai, Stephanie;Le Trionnaire, Gael;Bonhomme, Joel;Christophides, Georges K;Rispe, Claude;Tagu, Denis
通讯作者：
Tagu, Denis
影响因子：
2.2
作者：
Allen, Margaret L.;Walker, William B., III
通讯作者：
Walker, William B., III
影响因子：
2.6
作者：
Assis de Carvalho, Renato;Torres, Tatiana Teixeira;Lima de Azeredo-Espin, Ana Maria
通讯作者：
Lima de Azeredo-Espin, Ana Maria
DOI：
10.1098/rstb.1998.0320
发表时间：
1998-10-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Hemingway, J;Hawkes, N;Ranson, H
通讯作者：
Ranson, H
影响因子：
12.3
作者：
通讯作者：
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