Antagonistic Interaction of Staphylococcus aureus Toward Candida glabrata During in vitro Biofilm Formation Is Caused by an Apoptotic Mechanism.
Antagonistic Interaction of Staphylococcus aureus Toward Candida glabrata During in vitro Biofilm Formation Is Caused by an Apoptotic Mechanism.
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DOI：
10.3389/fmicb.2018.02031
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发表时间：
2018
影响因子：
5.2
通讯作者：
Rodríguez-Tovar AV
中科院分区：
文献类型：
--
作者：
Camarillo-Márquez O;Córdova-Alcántara IM;Hernández-Rodríguez CH;García-Pérez BE;Martínez-Rivera MA;Rodríguez-Tovar AV
背景：念珠菌属和金黄色葡萄球菌引起的感染与生物膜形成有关。C.白色链霉菌S.金黄色葡萄球菌的相互作用是协同的，这是由于混合生物膜的显著增加和对万古霉素的耐药性的改善。金黄色。C. glabrata和S.金黄色葡萄球菌都是在相似的宿主生态位中引起机会性感染的医院病原体。然而，关于这些最后的微生物之间的相互作用的信息很少。结果：C. glabrata和S.通过评估两种微生物在共生细胞的共培养物中以及在单一和混合生物膜中的生存力来评价金黄色葡萄球菌。S.金黄色葡萄球菌及其无细胞细菌上清液（CFBS）对C.光滑藻以浮游形式和生物膜形式存在。扫描电子显微镜（SEM），透射电子显微镜（TEM），和共聚焦激光扫描显微镜（CLSM）的图像显示酵母细胞被细菌包围，胞质膜内的变化，和无活力的芽生孢子与完整的细胞壁。同时，S。金黄色葡萄球菌细胞保持存活且未改变。S. aureus对C. glabrata不是由于细胞与细胞接触，而是由于CFBS的存在，CFBS导致酵母活力显著下降，形成大量脂滴（LD），活性氧（ROS）积累，以及核改变和DNA片段化，表明诱导凋亡机制。结论：我们的结果表明，S。aureus CFBS引起C. glabrata通过凋亡机制。
Background: Infections caused by Candida species and Staphylococcus aureus are associated with biofilm formation. C. albicans–S. aureus interactions are synergistic due to the significant increase in mixed biofilms and improved resistance to vancomycin of S. aureus. C. glabrata and S. aureus both are nosocomial pathogens that cause opportunistic infections in similar host niches. However, there is scarce information concerning the interaction between these last microorganisms. Results: The relationship between C. glabrata and S. aureus was evaluated by estimating the viability of both microorganisms in co-culture of planktonic cells and in single and mixed biofilms. An antagonistic behavior of S. aureus and their cell-free bacterial supernatant (CFBS) toward C. glabrata, both in planktonic form and in biofilms, was demonstrated. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM) images showed yeast cells surrounded by bacteria, alterations in intracytoplasmic membranes, and non-viable blastoconidia with intact cell walls. Concomitantly, S. aureus cells remained viable and unaltered. The antagonistic activity of S. aureus toward C. glabrata was not due to cell-to-cell contact but the presence of CFBS, which causes a significant decrement in yeast viability and the formation of numerous lipid droplets (LDs), reactive oxygen species (ROS) accumulation, as well as nuclear alterations, and DNA fragmentation indicating the induction of an apoptotic mechanism. Conclusion: Our results demonstrate that the S. aureus CFBS causes cell death in C. glabrata by an apoptotic mechanism.
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影响因子：
3.4
作者：
Dixon EF;Hall RA
通讯作者：
Hall RA
影响因子：
6.4
作者：
Arendrup, Maiken Cavling;Patterson, Thomas F.
通讯作者：
Patterson, Thomas F.
影响因子：
5.5
作者：
Kairo, SK;Bedwell, J;Corbel, MJ
通讯作者：
Corbel, MJ
DOI：
10.1073/pnas.1523199113
发表时间：
2016-06-28
影响因子：
11.1
作者：
Bosi, Emanuele;Monk, Jonathan M.;Palsson, Bernhard O.
通讯作者：
Palsson, Bernhard O.
影响因子：
4.9
作者：
Harriott, Melphine M.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.