MACC1 as a prognostic biomarker for early-stage and AFP-normal hepatocellular carcinoma.
MACC1 as a prognostic biomarker for early-stage and AFP-normal hepatocellular carcinoma.
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MACC1 作为早期和 AFP 正常肝细胞癌的预后生物标志物
DOI：
10.1371/journal.pone.0064235
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Song L
中科院分区：
文献类型：
--
作者：
Xie C;Wu J;Yun J;Lai J;Yuan Y;Gao Z;Li M;Li J;Song L
结肠癌转移相关基因1 （MACC1）已被发现与癌症的发生和进展有关。本研究的目的是探讨MACC1在早期和afp正常肝细胞癌（HCC）中的预后价值。方法采用逆转录- pcr和Western blot方法检测1例正常肝上皮细胞THLE3和15例肝癌细胞株中MACC1 mRNA和蛋白的表达水平。我们还比较研究了MACC1在6对HCC病变和相邻非癌组织样本中的表达。应用免疫组织化学方法分析308例临床病理特征的HCC中MACC1的表达。应用统计学分析得出MACC1表达评分与临床分期和患者生存之间的关系。结果肝癌细胞系和肝癌病变中MACC1 mRNA和蛋白水平高于正常肝上皮细胞和配对的邻近非癌组织。不同TNM分期患者MACC1表达差异有统计学意义（P<0.001）。总体生存分析显示，MACC1表达水平高与生存率低相关（P = 0.001）。重要的是，在早期HCC患者或血清AFP水平正常的患者中，MACC1水平与患者生存率呈显著负相关。结论MACC1蛋白可能是预测HCC预后的一个有希望的生物标志物，包括早期和afp正常患者。
Background The metastasis-associated in colon cancer 1 gene (MACC1) has been found to be associated with cancer development and progression. The aim of this study was to investigate the prognostic value of MACC1 in early-stage and AFP-normal hepatocellular carcinoma (HCC). Methods mRNA and protein levels of MACC1 expression in one normal liver epithelial cells THLE3 and 15 HCC cell lines were examined using reverse transcription-PCR and Western blot. MACC1 expression was also comparatively studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Immunohistochemistry was employed to analyze MACC1 expression in 308 clinicopathologically characterized HCC cases. Statistical analyses were applied to derive association between MACC1 expression scores and clinical staging as well as patient survival. Results Levels of MACC1 mRNA and protein were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. Significant difference in MACC1 expression was found in patients of different TNM stages (P<0.001). Overall survival analysis showed that high MACC1 expression level correlated with lower survival rate (P = 0.001). Importantly, an inverse correlation between MACC1 level and patient survival remained significant in subjects with early-stage HCC or with normal serum AFP level. Conclusions MACC1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.
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人肝细胞癌的染色体改变与探索性CGH荟萃分析的病因学和组织学等级相关。
影响因子：
8.8
作者：
Moinzadeh, P;Breuhahn, K;Stützer, H;Schirmacher, P
通讯作者：
Schirmacher, P
DOI：
10.1111/j.1478-3231.2008.01957.x
发表时间：
2009-04
期刊：
Liver international : official journal of the International Association for the Study of the Liver
影响因子：
--
作者：
Tandon P;Garcia-Tsao G
通讯作者：
Garcia-Tsao G
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
15.9
作者：
Kaposi-Novak, Pal;Lee, Ju-Seog;Thorgeirsson, Snorri S.
通讯作者：
Thorgeirsson, Snorri S.
MACC1 鉴定为肝细胞癌的新型预后标志物
影响因子：
7.4
作者：
Qiu J;Huang P;Liu Q;Hong J;Li B;Lu C;Wang L;Wang J;Yuan Y
通讯作者：
Yuan Y