Global expression profiling in leaves of free-growing aspen.
Global expression profiling in leaves of free-growing aspen.
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DOI：
10.1186/1471-2229-8-61
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发表时间：
2008-05-23
影响因子：
5.3
通讯作者：
Jansson S
中科院分区：
文献类型：
--
作者：
Sjödin A;Wissel K;Bylesjö M;Trygg J;Jansson S
基因组研究通常在与自然条件非常不同的受控环境中对幼苗进行。事实上，温带国家的植物在环境条件下会受到很大的波动，多年生植物会在几年内受到影响。我们已经研究了在多个生长季节的自由生长的白杨（杨树tremula）的叶片中的基因表达。我们表明，在叶片发育的第一个月期间的基因表达在很大程度上是由一个发育程序决定的，尽管叶片扩展，叶绿素积累和通过这个程序的进展速度是由温度调节的。我们还能够定义四个不同的叶发育亚阶段的“转录签名”。在成熟叶片中，气候因子对基因调控起重要作用。这项研究表明，多变量方法与高通量转录方法在该领域可以提供额外的，新的信息，植物状态在不断变化的环境条件下，是不可能在实验室条件下模拟。我们已经生成了一个数据集，可用于例如识别某些发育阶段或治疗的标记基因，以及评估基因表达的自然变异。
Genomic studies are routinely performed on young plants in controlled environments which is very different from natural conditions. In reality plants in temperate countries are exposed to large fluctuations in environmental conditions, in the case of perennials over several years. We have studied gene expression in leaves of a free-growing aspen (Populus tremula) throughout multiple growing seasons We show that gene expression during the first month of leaf development was largely determined by a developmental program although leaf expansion, chlorophyll accumulation and the speed of progression through this program was regulated by the temperature. We were also able to define "transcriptional signatures" for four different substages of leaf development. In mature leaves, weather factors were important for gene regulation. This study shows that multivariate methods together with high throughput transcriptional methods in the field can provide additional, novel information as to plant status under changing environmental conditions that is impossible to mimic in laboratory conditions. We have generated a dataset that could be used to e.g. identify marker genes for certain developmental stages or treatments, as well as to assess natural variation in gene expression.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
12.3
作者：
Andersson A;Keskitalo J;Sjödin A;Bhalerao R;Sterky F;Wissel K;Tandre K;Aspeborg H;Moyle R;Ohmiya Y;Bhalerao R;Brunner A;Gustafsson P;Karlsson J;Lundeberg J;Nilsson O;Sandberg G;Strauss S;Sundberg B;Uhlen M;Jansson S;Nilsson P
通讯作者：
Nilsson P
影响因子：
7.3
作者：
Cookson, SJ;Van Lijsebettens, M;Granier, C
通讯作者：
Granier, C
影响因子：
6.9
作者：
Brandt, S;Kloska, S;Kehr, J
通讯作者：
Kehr, J
影响因子：
7.4
作者：
Beemster, GTS;De Veylder, L;Vuylsteke, M
通讯作者：
Vuylsteke, M