The Expression and Anatomical Distribution of BTLA and Its Ligand HVEM in Rheumatoid Synovium
The Expression and Anatomical Distribution of BTLA and Its Ligand HVEM in Rheumatoid Synovium
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BTLA及其配体HVEM在类风湿滑膜中的表达和解剖分布
DOI：
10.1007/s10753-011-9417-2
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发表时间：
2012-06
期刊：
影响因子：
5.1
通讯作者：
Chen, Yongwen
中科院分区：
文献类型：
--
作者：
Shang, Yongjun;Guo, Guoning;Cui, Qifu;Li, Jinlong;Ruan, Zhihua;Chen, Yongwen
来自B和T淋巴细胞衰减剂（BTLA）的共抑制信号可以抑制淋巴细胞活化并维持外周耐受。然而，BTLA及其配体，疱疹病毒进入介质（HVEM），在类风湿关节炎（RA）滑膜的表达和解剖分布还没有报道。在这项研究中，我们分析了HVEM和BTLA在RA滑膜免疫组化的表达，我们的结果表明，这两个因素都观察到在所有四例RA样本。在细胞水平上，HVEM和BTLA均存在于细胞膜和细胞质中。荧光双染显示HVEM主要分布在CD 3 +T细胞、CD 68+巨噬细胞上，CD 31+内皮细胞上也有少量表达。同样，在浸润的CD 3 +T细胞和CD 68+巨噬细胞上观察到BTLA的表达。结果表明，HVEM抗原在B7-H3+毛细血管中也有表达，而在B7-H1+、B7-DC+、B7-H4+和Z39 Ig+细胞中均无表达。有趣的是，在滑膜中的B7-H1+、B7-H4+和HVEM+细胞上观察到BTLA。BTLA/HVEM在滑膜中的特征性表达和分布表明，它们的信号传导可能影响RA的发病机制，对其功能作用的明确认识将有助于进一步阐明RA的发病机制。
Co-inhibitory signaling from B and T lymphocyte attenuator (BTLA) can suppress lymphocyte activation and maintain peripheral tolerance. However, the expression and anatomical distribution of BTLA and its ligand, herpesvirus entry mediator (HVEM), in rheumatoid arthritis (RA) synovium have not been reported. In this study, we analyzed the expression of HVEM and BTLA in RA synovium by immunohistochemistry, and our results showed that both factors were observed in all four cases of RA samples. At the cellular level, both HVEM and BTLA were found on the cell membrane and in the cytoplasm. Fluorescence dual staining demonstrated that HVEM was chiefly on CD3+T cells, CD68+macrophages, and to a lesser extent was found on CD31+endothelial cells. Similarly, the expression of BTLA was observed on infiltrated CD3+T cells and CD68+macrophages. The co-expression of HVEM and BTLA with some members of the B7 family in these sections was also analyzed, and the results showed that HVEM antigen was also found on B7-H3+capillaries, while it was absent on B7-H1+, B7-DC+, B7-H4+, and Z39Ig+cells. Interestingly, BTLA was observed on B7-H1+, B7-H4+, and HVEM+cells in the synovium. The characteristic expression and distribution of BTLA/HVEM in the synovium indicated that their signaling probably affects the pathogenesis of RA, and a clear understanding of their functional roles may further elucidate the pathogenesis of this disease.
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DOI：
10.1084/jem.20071160
发表时间：
2008-06-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Steinberg MW;Turovskaya O;Shaikh RB;Kim G;McCole DF;Pfeffer K;Murphy KM;Ware CF;Kronenberg M
通讯作者：
Kronenberg M
DOI：
10.1084/jem.20070432
发表时间：
2007-06-11
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Katschke KJ Jr;Helmy KY;Steffek M;Xi H;Yin J;Lee WP;Gribling P;Barck KH;Carano RA;Taylor RE;Rangell L;Diehl L;Hass PE;Wiesmann C;van Lookeren Campagne M
通讯作者：
van Lookeren Campagne M
DOI：
10.1073/pnas.0902115106
发表时间：
2009-04-14
影响因子：
11.1
作者：
Cheung, Timothy C.;Steinberg, Marcos W.;Ware, Carl F.
通讯作者：
Ware, Carl F.
影响因子：
4
作者：
Wang, Guohua;Hu, Ping;Wu, Xiongwen
通讯作者：
Wu, Xiongwen
影响因子：
--
作者：
Oya, Yoshihiro;Watanabe, Norihiko;Owada, Takayoshi;Oki, Mie;Hirose, Koichi;Suto, Akira;Kagami, Shin-ichiro;Nakajima, Hiroshi;Kishimoto, Takashi;Iwamoto, Itsuo;Murphy, Theresa L.;Murphy, Kenneth M.;Saito, Yasushi
通讯作者：
Saito, Yasushi