Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device
Amperometric detection of DNA hybridization using a multi-point, addressable electrochemical device
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使用多点、可寻址电化学装置对 DNA 杂交进行电流检测
DOI：
10.1016/j.snb.2011.09.004
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发表时间：
2011-12
影响因子：
8.4
通讯作者：
Matsue, Tomokazu
中科院分区：
文献类型：
--
作者：
Zhu, Xi;Ino, Kosuke;Lin, Zhenyu;Shiku, Hitoshi;Chen, Guonan;Matsue, Tomokazu
设计了一种新的DNA杂交多点寻址检测装置。将行电极阵列和列电极阵列正交排列，将微孔组装在行/列电极的交叉点上，形成4×4的微孔阵列。基于电极之间发生的局部电活性物质的氧化还原循环，可以分别检测各个微孔处的安培信号。固定化和杂交可以阻断Fe(CN)−/Fe(CN)63−在指定的微孔处的氧化还原循环，导致电流响应降低。该装置用于DNA杂交检测，具有良好的灵敏度(0.03μM)和选择性。该装置可应用于生化物种的全面、高通量检测和成像。
A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4×4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)64−/Fe(CN)63−at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03μM) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species.
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影响因子：
12.6
作者：
B. Lillis;M. Manning;Eileen Hurley;H. Berney;R. Duane;A. Mathewson;M. Sheehan
通讯作者：
B. Lillis;M. Manning;Eileen Hurley;H. Berney;R. Duane;A. Mathewson;M. Sheehan
影响因子：
12.6
作者：
O. Henry;J. L. A. Sanchez;D. Latta;C. O’Sullivan
通讯作者：
O. Henry;J. L. A. Sanchez;D. Latta;C. O’Sullivan
影响因子：
7.4
作者：
Wolfrum, Bernhard;Zevenbergen, Marcel;Lemay, Serge
通讯作者：
Lemay, Serge
影响因子：
12.6
作者：
Dongdong Zhang;Yage Peng;H. Qi;Q. Gao;Chengxiao Zhang
通讯作者：
Dongdong Zhang;Yage Peng;H. Qi;Q. Gao;Chengxiao Zhang
基于Ru(bpy)32氧化还原循环的可寻址电化学发光检测系统
影响因子：
4.9
作者：
Lin, Zhenyu;Ino, Kosuke;Chen, Guonan
通讯作者：
Chen, Guonan